Pan-myeloid Differentiation of Human Cord Blood Derived CD34+ Hematopoietic Stem and Progenitor Cells

被引：5

作者：

Bapat, Aditi ^{[1
]}

Keita, Nakia ^{[1
]}

Sharma, Shalini ^{[1
]}

机构：

[1] Univ Arizona, Coll Med Phoenix, Dept Basic Med Sci, Phoenix, AZ 85004 USA

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2019年 / 150期

基金：

美国国家卫生研究院;

关键词：

Developmental Biology; Issue; 150; CD34(+) cells; myeloid differentiation; hematopoietic stem and progenitor cells; granulocyte; monocyte; erythrocyte; megakaryocyte; common myeloid progenitor; granulocyte monocyte progenitor; megakaryocyte erythroid progenitor; flow cytometry; SELF-RENEWAL; BONE-MARROW; IDENTIFICATION;

D O I：

10.3791/59836

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ex vivo differentiation of human hematopoietic stem cells is a widely used model for studying hematopoiesis. The protocol described here is for cytokine induced differentiation of CD34(+) hematopoietic stem and progenitor cells to the four myeloid lineage cells. CD34(+) cells are isolated from human umbilical cord blood and co-cultured with MS-5 stromal cells in the presence of cytokines. Immunophenotypic characterization of the stem and progenitor cells, and the differentiated myeloid lineage cells are described. Using this protocol, CD34(+) cells may be incubated with small molecules or transduced with lentiviruses to express myeloid disease mutations to investigate their impact on myeloid differentiation.

引用

页数：9

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