NMR structure determination of protein-ligand complexes by lanthanide labeling

被引：218

作者：

Pintacuda, Guido ^{[1
]}

John, Michael ^{[1
]}

Su, Xun-Cheng ^{[1
]}

Otting, Gottfried ^{[1
]}

机构：

[1] Australian Natl Univ, Res Sch Chem, Canberra, ACT 0200, Australia

来源：

ACCOUNTS OF CHEMICAL RESEARCH | 2007年 / 40卷 / 03期

关键词：

D O I：

10.1021/ar050087z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The paramagnetism of lanthanide ions offers outstanding opportunities for fast determinations of the three-dimensional (3D) structures of protein-ligand complexes by nuclear magnetic resonance (NMR) spectroscopy. It is shown how the combination of pseudocontact shifts (PCSs) induced by a site-specifically bound lanthanide ion and prior knowledge of the 3D structure of the lanthanide-labeled protein can be used to achieve (i) rapid assignments of NMR spectra, (ii) structure determinations of protein-protein complexes, and (iii) identification of the binding mode of low-molecular weight compounds in complexes with proteins. Strategies for site-specific incorporation of lanthanide ions into proteins are summarized.

引用

页码：206 / 212

页数：7

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