An Allosteric Dual-DNAzyme Unimolecular Probe for Colorimetric Detection of Copper(II)

被引：273

作者：

Yin, Bin-Cheng ^{[1
]}

Ye, Bang-Ce ^{[1
]}

Tan, Weihong ^{[2
,3
]}

Wang, Hui ^{[2
,3
]}

Xie, Cong-Cong ^{[1
]}

机构：

[1] E China Univ Sci & Technol, Lab Biosyst & Microanal, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

[2] Univ Florida, Dept Chem & Physiol, Ctr Res Bio Nano Interface, Gainesville, FL 32611 USA

[3] Univ Florida, Shands Canc Ctr, Gainesville, FL 32611 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2009年 / 131卷 / 41期

关键词：

IN-VITRO SELECTION; CLEAVING DEOXYRIBOZYME; DNA; IONS; LEAD; SENSOR; SENSITIVITY;

D O I：

10.1021/ja9062426

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

An effective dual-DNAzyme-based unimolecular probe design employing intramolecular signal transduction is demonstrated. The probe is composed of three domains: a DNA-cleaving DNAzyme, a substrate, and an HRP-mimicking DNAzyme. When the probe meets its target, cleavage of the substrate by the DNA-cleaving DNAzyme activates the HRP-mimicking DNAzyme, producing a colorimetric signal. The Cu2+-dependent DNAzyme engineered to demonstrate this design revealed a sensitivity corresponding to 65 ppb, which is sufficient to detect Cu2+ in drinking water. The new probe has excellent selectivity toward Cu2+. This three-component design is simple and easy to engineer. It may provide the basis for future development of other nucleic acid-based probes for toxicological and environmental monitoring.

引用

页码：14624 / +

页数：3

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