Structural determination of the conjugate of human serum albumin with a mitomycin C derivative, KW-2149, by matrix-assisted laser desorption/ionization mass spectrometry

被引：16

作者：

Yasuzawa, T ^{[1
]}

Tomer, KB ^{[1
]}

机构：

[1] NIEHS,STRUCT BIOL LAB,RES TRIANGLE PK,NC 27709

来源：

BIOCONJUGATE CHEMISTRY | 1997年 / 8卷 / 03期

关键词：

D O I：

10.1021/bc970038+

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

new mitomycin C derivative, KW-2149, is known to form a covalent conjugate with human serum albumin (HSA). This conjugate exhibits 1/20 of the anticellular activity of unconjugated KW-2149. Structural studies of this conjugate were carried out using a combination of enzymatic digestion, high-performance liquid chromatography (HPLC), and matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The tryptic peptide T5 (residues 21-41) was the only peptide found to be modified by KW-2149 moieties, the [(gamma-L-glutamylamino)ethyl]thio group or the (2-aminoethyl)thio group, through a disulfide bond. Although the latter peptide lost its mitomycin C moiety in the course of tryptic digestion, these data strongly suggest that KW-2149 was bound to Cys-34, the only free cysteine on KSA.

引用

页码：391 / 399

页数：9

共 35 条

[1] REDUCED BONE-MARROW TOXICITY OF KW-2149, A MITOMYCIN-C DERIVATIVE, IN MICE [J].