Purification and characterization of two isozymes of polygalacturonase from Botrytis cinerea.: Effect of calcium ions on polygalacturonase activity

被引：36

作者：

Cabanne, C ^{[1
]}

Donèche, B ^{[1
]}

机构：

[1] Univ Bordeaux 2, Lab Biochim Vegetale Appl, Fac Oenol, INRA, F-33405 Talence, France

来源：

MICROBIOLOGICAL RESEARCH | 2002年 / 157卷 / 03期

关键词：

Botrytis cinerea; polygalacturonase; calcium; pectin;

D O I：

10.1078/0944-5013-00147

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The phytopathogenic fungus Botrytis cinerea produces a set of polygalacturonases (PGs) which are involved in the enzymatic degradation of pectin during plant tissue infection. Two polygalacturonases secreted by B. cinerea in seven-day-old liquid culture were purified to apparent homogeneity by chromatography. PG I was an exopolygalacturonase of molecular weight 65 kDa and pI 8.0 and PG II was an endopolygalacturonase of 52 kDa and pI 7.8. Enzymatic activity of PG I and PG II was partially inhibited by 1 mM CaCl2, probably by calcium chelation of polygalacturonic acid, the substrate of the enzyme.

引用

页码：183 / 189

页数：7

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