Flavone polyphenols dominate in Thymus schimperi Ronniger: LC-ESI-MS/MS characterization and study of anti-proliferative effects of plant extract on AGS and HepG2 cancer cells

被引:35
作者
Desta, Kebede Taye [1 ,2 ]
Kim, Gon Sup [3 ,4 ]
Abd El-Aty, A. M. [5 ,6 ]
Raha, Suchismita [3 ,4 ]
Kim, Man-Bae [7 ]
Jeong, Ji Hoon [8 ]
Warda, Mohamad [9 ]
Hacimuftuoglu, Ahmet [10 ]
Shin, Ho-Chul [5 ]
Shim, Jae-Han [11 ]
Shin, Sung Chul [1 ,2 ]
机构
[1] Gyeongsang Natl Univ, Dept Chem, Jinju 660701, South Korea
[2] Gyeongsang Natl Univ, Res Inst Life Sci, Jinju 660701, South Korea
[3] Gyeongsang Natl Univ, Res Inst Life Sci, Jinju 660701, South Korea
[4] Gyeongsang Natl Univ, Coll Vet Med, Jinju 660701, South Korea
[5] Konkuk Univ, Coll Vet Med, Dept Vet Pharmacol & Toxicol, 1 Hwayang Dong, Seoul 143701, South Korea
[6] Cairo Univ, Fac Vet Med, Dept Pharmacol, Giza 12211, Egypt
[7] Gyeongsangnam Do Agr Res & Extens Serv, Jinju 660985, South Korea
[8] Chung Ang Univ, Coll Med, Dept Pharmacol, 221 Heuksuk Dong, Seoul 156756, South Korea
[9] Cairo Univ, Fac Vet Med, Biochem & Chem Nutr Dept, Giza 12211, Egypt
[10] Ataturk Univ, Med Fac, Dept Med Pharmacol, Erzurum, Turkey
[11] Chonnam Natl Univ, Coll Agr & Life Sci, Nat Prod Chem Lab, 77 Yongbong Ro, Gwangju 500757, South Korea
来源
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 2017年 / 1053卷
基金
新加坡国家研究基金会;
关键词
Thymus schimperi; Polyphenols; Isolation; Tandem mass spectrometry; Anti-proliferative effects; Cancer cells; TANDEM MASS-SPECTROMETRY; CAPSICUM-ANNUUM L; HPLC-DAD; PHENOLIC-COMPOUNDS; ANTIOXIDANT; IDENTIFICATION; CONSTITUENTS; VULGARIS; LEAVES; FOOD;
D O I
10.1016/j.jchromb.2017.03.035
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Thymus schimperi is a highly localized and a rare plant endemic to Ethiopia. An optimized and validated high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) method was applied to characterize 23 polyphenolic compounds found in ethyl acetate extracts of the plant. From those, flavones dominated and luteolin was the major component contributing 21.83% of the total composition (or 46.05 +/- 0.59 g/kg of fresh sample weight). Validation data showed a determination coefficient (R-2) >= 0.997. Limits of detection (LOD) and quantification (LOQ) were 0.03-0.97 and 0.11-3.23 mg/L, while recovery values spiked at 5 and 50 mg/L were between 70.89-115.39 and 67.65-120.19%, respectively. Except for caffeic acid and epicatechin gallate, the relative standard deviations (%RSD5) were far below 15%, showing acceptable precision values. The plant extracts inhibited cell proliferation and induced cell death in human gastric adenocarcinoma (AGS) and liver hepatocellular carcinoma (HepG2) cancer cells. This is the first report of polyphenolic components from T. schimperi being characterized using HPLC-ESI-MS/MS. Being components of many edible vegetables, fruits, and spices, the identified polyphenols suggest that T. schimperi could be a potential food with promising health benefits. (C) 2017 Elsevier B.V. All rights reserved.
引用
收藏
页码:1 / 8
页数:8
相关论文
共 57 条
[1]   New features on the fragmentation and differentiation of C-glycosidic flavone isomers by positive electrospray ionization and triple quadrupole mass spectrometry [J].
Abad-Garcia, Beatriz ;
Garmon-Lobato, Sergio ;
Berrueta, Luis A. ;
Gallo, Blanca ;
Vicente, Francisca .
RAPID COMMUNICATIONS IN MASS SPECTROMETRY, 2008, 22 (12) :1834-1842
[2]   CHROMATOGRAPHIC ANALYSIS OF POLYPHENOLS OF SOME IBERIAN THYMUS [J].
ADZET, T ;
VILA, R ;
CANIGUERAL, S .
JOURNAL OF ETHNOPHARMACOLOGY, 1988, 24 (2-3) :147-154
[3]   Polyphenolic Profile of Pear Leaves with Different Resistance to Pear Psylla (Cacopsylla pyri) [J].
Aksic, Milica M. Fotiric ;
Dabic, Dragana C. ;
Gasic, Uros M. ;
Zec, Gordan N. ;
Vulic, Todor B. ;
Tesic, Zivoslav Lj. ;
Natic, Maja M. .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2015, 63 (34) :7476-7486
[4]   HIGHLY METHYLATED 6-HYDROXYFLAVONES AND OTHER FLAVONOIDS FROM THYMUS-PIPERELLA [J].
BARBERAN, FAT ;
HERNANDEZ, L ;
FERRERES, F ;
TOMAS, F .
PLANTA MEDICA, 1985, 51 (05) :452-454
[5]   Kinetic optimisation of the reversed phase liquid chromatographic separation of rooibos tea (Aspalathus linearis) phenolics on conventional high performance liquid chromatographic instrumentation [J].
Beelders, Theresa ;
Sigge, Gunnar O. ;
Joubert, Elizabeth ;
de Beer, Dalene ;
de Villiers, Andre .
JOURNAL OF CHROMATOGRAPHY A, 2012, 1219 :128-139
[6]   Determination of polyphenolic compounds by liquid chromatography-mass spectrometry in Thymus species [J].
Boros, Borbala ;
Jakabova, Silvia ;
Doernyei, Agnes ;
Horvath, Gyoergyi ;
Pluhar, Zsuzsanna ;
Kilar, Ferenc ;
Felinger, Attila .
JOURNAL OF CHROMATOGRAPHY A, 2010, 1217 (51) :7972-7980
[7]   Effects of Thymus serpyllum Extract on Cell Proliferation, Apoptosis and Epigenetic Events in Human Breast Cancer Cells [J].
Bozkurt, Emir ;
Atmaca, Harika ;
Kisim, Asli ;
Uzunoglu, Selim ;
Uslu, Ruchan ;
Karaca, Burcak .
NUTRITION AND CANCER-AN INTERNATIONAL JOURNAL, 2012, 64 (08) :1245-1250
[8]   The green tea molecule EGCG inhibits Zika virus entry [J].
Carneiro, Bruno M. ;
Batista, Mariana N. ;
Braga, Ana Claudia S. ;
Nogueira, Mauricio L. ;
Rahal, Paula .
VIROLOGY, 2016, 496 :215-218
[9]   Analysis of Flavonoids in Rhamnus davurica and Its Antiproliferative Activities [J].
Chen, Guilin ;
Li, Xun ;
Saleri, Flora ;
Guo, Mingquan .
MOLECULES, 2016, 21 (10)
[10]   Qualitative Analysis and Simultaneous Quantification of Phenolic Compounds in the Aerial Parts of Salvia miltiorrhiza by HPLC-DAD and ESI/MSn [J].
Chen, Hong ;
Zhang, Qin ;
Wang, Xiaoming ;
Yang, Jie ;
Wang, Qiang .
PHYTOCHEMICAL ANALYSIS, 2011, 22 (03) :247-257