Aflatoxin M1 determination and stability study in milk samples using a screen-printed 96-well electrochemical microplate

被引：31

作者：

Neagu, D. ^{[1
]}

Perrino, S. ^{[1
]}

Micheli, L. ^{[1
]}

Palleschi, G. ^{[1
]}

Moscone, D. ^{[1
]}

机构：

[1] Univ Roma Tor Vergata, Dipartimento Sci & Technol Chim, I-00133 Rome, Italy

来源：

INTERNATIONAL DAIRY JOURNAL | 2009年 / 19卷 / 12期

关键词：

CHEESE SAMPLES; IMMUNOSENSOR; MYCOTOXINS; PRODUCTS; M(1); M1;

D O I：

10.1016/j.idairyj.2009.06.004

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

A highly sensitive method for the determination of aflatoxin M-1 (AFM(1)) is described that involves the use of a disposable multichannel microplate coupled to intermittent pulse amperometry (IPA) for immunosensor development based on a direct competitive assay. Immunoassay parameters were evaluated and optimized to establish an electrochemical enzyme-linked immunosorbent assay (ELISA) procedure for AFM(1) in milk samples. The suitability of the immunosensors for the direct analysis of the toxin in milk was assessed. AFM(1) was measured with a working range of 5-250 pg mL(-1) and a detection limit of 1 pg mL(-1). Good recovery values (90-105%) were obtained. The method was used to study the recovery of this toxin in frozen (-30 degrees C) or lyophilized (4 degrees C) milk samples stored for up to 3 months. A significant, but variable, decrease (>50%) of the measured AFM(1) concentration with respect to the initial toxin contamination levels was found. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：753 / 758

页数：6

共 30 条

[21] An electrochemical immunosensor for aflatoxin M1 determination in milk using screen-printed electrodes [J].

Micheli, L ;

Grecco, R ;

Badea, M ;

Moscone, D ;

Palleschi, G .

BIOSENSORS & BIOELECTRONICS, 2005, 21 (04) :588-596

[22]

Peraica M, 1999, B WORLD HEALTH ORGAN, V77, P754

[23] Electrochemical immunosensor array using a 96-well screen-printed microplate for aflatoxin B1 detection [J].

Piermarini, S. ;

Micheli, L. ;

Ammida, N. H. S. ;

Palleschi, G. ;

Moscone, D. .

BIOSENSORS & BIOELECTRONICS, 2007, 22 (07) :1434-1440

[24] Detection of aflatoxin M1 in cheese samples by ELISA [J].

Sarimehmetoglu, B ;

Kuplulu, O ;

Celik, TH .

FOOD CONTROL, 2004, 15 (01) :45-49

[25]

*STAND COMM FOODS, 1994, 94 M SCF BRUSS BELG

[26] STABILITY OF AFLATOXIN-M IN MILK [J].

STOLOFF, L ;

TRUCKSESS, M ;

HARDIN, N ;

FRANCIS, OJ ;

HAYES, JR ;

POLAN, CE ;

CAMPBELL, TC .

JOURNAL OF DAIRY SCIENCE, 1975, 58 (12) :1789-1793

[27]

WARWICK MJ, 1996, IMMUNOASSAY PRACTICA, P150

[28]

Wojciechowski M, 1999, CLIN CHEM, V45, P1690

[29]

Wood G. E., 1991, Mycotoxins and phytoalexins., P145

[30] Aflatoxin M1 levels in cheese samples from some provinces of Turkey [J].

Yaroglu, T ;

Oruc, HH ;

Tayar, M .

FOOD CONTROL, 2005, 16 (10) :883-885

← 1 2 3 →