Effects of meropenem exposure in persister cells of Acinetobacter calcoaceticus-baumannii

被引：11
|
作者：
Gallo, Stephanie Wagner [1 ]
Donamore, Bruna Kern [1 ]
Pagnussatti, Vany Elisa [2 ]
Sanchez Ferreira, Carlos Alexandre [1 ]
de Oliveira, Silvia Dias [1 ]
机构：
[1] Pontificia Univ Catolica Rio Grande do Sul PUCRS, Lab Imunol & Microbiol, Fac Biociencias, Porto Alegre, RS, Brazil
[2] Pontificia Univ Catolica Rio Grande do Sul PUCRS, Dept Microbiol, Lab Patol Clin, Hosp Sao Lucas, Porto Alegre, RS, Brazil
来源：
FUTURE MICROBIOLOGY | 2017年 / 12卷 / 02期
关键词：
carbapenems; chronic infections; persistence; therapeutic failure; PSEUDOMONAS-AERUGINOSA; STAPHYLOCOCCUS-AUREUS; ANTIBIOTIC TOLERANCE; MULTIDRUG TOLERANCE; OXIDATIVE STRESS; BIOFILMS; RESISTANCE; TARGET;
D O I：
10.2217/fmb-2016-0118
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
Aim: To evaluate the influence of meropenem in the Acinetobacter calcoaceticus-baumannii (ACB) persister levels. Methods: Persister levels in planktonic and biofilm cultures from ACB isolates were evaluated after exposure to different meropenem concentrations. Results: A high variability of persister fractions was observed among the isolates cultured under planktonic and biofilm conditions. Meropenem concentration did not influence persister fractions, even when far above the MIC. No correlation was found between persister levels and biofilm biomass. Conclusion: The magnitude of persister levels from ACB planktonic and, particularly, biofilm cultures exposed to meropenem was independent of the antibiotic concentration, dosing regimen and biofilm biomass. These findings, in a context of meropenem failure to treat chronic infections, strengthen the importance of understanding persister behavior.
引用
收藏
页码：131 / 140
页数：10
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