Validation of a rapid DNA process with the RapidHIT® ID system using GlobalFiler® Express chemistry, a platform optimized for decentralized testing environments

被引：40

作者：

Salceda, Susana ^{[1
]}

Barican, Arnaldo ^{[1
]}

Buscaino, Jacklyn ^{[1
]}

Goldman, Bruce ^{[1
]}

Klevenberg, Jim ^{[1
]}

Kuhn, Melissa ^{[1
]}

Lehto, Dennis ^{[1
]}

Lin, Frank ^{[1
]}

Phong Nguyen ^{[1
]}

Park, Charles ^{[1
]}

Pearson, Francesca ^{[1
]}

Pittaro, Rick ^{[1
]}

Salodkar, Sayali ^{[1
]}

Schueren, Robert ^{[1
]}

Smith, Corey ^{[1
]}

Troup, Charles ^{[1
]}

Tsou, Dean ^{[1
]}

Vangbo, Mattias ^{[1
]}

Wunderle, Justus ^{[1
]}

King, David ^{[1
]}

机构：

[1] IntegenX Inc, 5720 Stoneridge Dr,Suite 300, Pleasanton, CA 94588 USA

来源：

FORENSIC SCIENCE INTERNATIONAL-GENETICS | 2017年 / 28卷

关键词：

SWGDAM developmental validation; Rapid DNA RapidHIT (R); RapidHIT (R) ID; RapidLINK (TM); GlobalFiler (R) Express; STR; Presumed single source sample; HUMAN IDENTIFICATION SYSTEM; DEVELOPMENTAL VALIDATION; KIT; PERFORMANCE; ASSAY; PCR;

D O I：

10.1016/j.fsigen.2017.01.005

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

The RapidHIT (R) ID is a fully automated sample-to-answer system for short tandem repeat (STR)-based human identification. The RapidHIT ID has been optimized for use in decentralized environments and processes presumed single source DNA samples, generating Combined DNA Index System (CODIS)compatible DNA profiles in less than 90 min. The system is easy to use, requiring less than one minute of hands-on time. Profiles are reviewed using centralized linking software, RapidLINK (TM) (IntegenX, Pleasanton, CA), a software tool designed to collate DNA profiles from single or multiple RapidHIT ID systems at different geographic locations. The RapidHIT ID has been designed to employ GlobalFiler (R) Express and AmpFLSTR (R) NGMSElect (TM), Thermo Fisher Scientific (Waltham, MA) STR chemistries. The Developmental Validation studies were performed using GlobalFiler (R) Express with single source reference samples according to Scientific Working Group for DNA Analysis Methods guidelines. These results show that multiple RapidHIT ID systems networked with RapidLINK software form a highly reliable system for wide-scale deployment in locations such as police booking stations and border crossings enabling real-time testing of arrestees, potential human trafficking victims, and other instances where rapid turnaround is essential. (C) 2017 The Author(s). Published by Elsevier Ireland Ltd.

引用

页码：21 / 34

页数：14

共 19 条

[1]

[Anonymous], PUB THERM SCI

[2] Evaluation of the RapidHIT™ 200 and RapidHIT GlobalFiler® Express kit for fully automated STR genotyping [J].

Date-Chong, Mavis ;

Hudlow, William R. ;

Buoncristiani, Martin R. .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2016, 23 :1-8

[3]

EDWARDS A, 1991, AM J HUM GENET, V49, P746

[4]

Federal Bureau of Investigation, 2011, QUAL ASS STAND QAS F

[5] DNA investigative lead development from blood and saliva samples in less than two hours using the RapidHIT(TM) Human DNA Identification System [J].

Gangano, Stefanie ;

Elliott, Keith ;

Anoruo, Kelechi ;

Gass, Jenny ;

Buscaino, Jacklyn ;

Jovanovich, Stevan ;

Harris, Dennis .

FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES, 2013, 4 (01) :E43-E44

[6] Developmental validation of the GlobalFiler® express kit, a 24-marker STR assay, on the RapidHIT® System [J].

Hennessy, Lori K. ;

Mehendale, Neelima ;

Chear, Kaiwan ;

Jovanovich, Stevan ;

Williams, Stephen ;

Park, Charles ;

Gangano, Stefanie .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2014, 13 :247-258

[7] Developmental validation studies on the RapidHIT(TM) Human DNA Identification System [J].

Hennessy, Lori K. ;

Franklin, Helen ;

Li, Yuan ;

Buscaino, Jacklyn ;

Chear, Kaiwan ;

Gass, Jennifer ;

Mehendale, Neelima ;

Williams, Stephen ;

Jovanovich, Stevan ;

Harris, Dennis ;

Elliott, Keith ;

Nielsen, William .

FORENSIC SCIENCE INTERNATIONAL GENETICS SUPPLEMENT SERIES, 2013, 4 (01) :E7-E8

[8] Evaluation of the RapidHIT™ 200, an automated human identification system for STR analysis of single source samples [J].

Holland, Mitchell ;

Wendt, Frank .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2015, 14 :76-85

[9] HYPERVARIABLE MINISATELLITE REGIONS IN HUMAN DNA [J].

JEFFREYS, AJ ;

WILSON, V ;

THEIN, SL .

NATURE, 1985, 314 (6006) :67-73

[10] Developmental validation of a fully integrated sample-to-profile rapid human identification system for processing single-source reference buccal samples [J].

Jovanovich, Stevan ;

Bogdan, Greg ;

Belcinski, Richard ;

Buscaino, Jacklyn ;

Burgi, Dean ;

Butts, Erica L. R. ;

Chear, Kaiwan ;

Ciopyk, Brian ;

Eberhart, David ;

El-Sissi, Omar ;

Franklin, Helen ;

Gangano, Stefanie ;

Gass, Jennifer ;

Harris, Dennis ;

Hennessy, Lori ;

Kindwall, Alex ;

King, David ;

Klevenberg, Jim ;

Li, Yuan ;

Mehendale, Neelima ;

McIntosh, Roger ;

Nielsen, Bill ;

Park, Charles ;

Pearson, Francesca ;

Schueren, Robert ;

Stainton, Nancy ;

Troup, Charles ;

Vallone, Peter M. ;

Vangbo, Mattias ;

Woudenberg, Timothy ;

Wyrick, David ;

Williams, Stephen .

FORENSIC SCIENCE INTERNATIONAL-GENETICS, 2015, 16 :181-194

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