Cloning, sequencing and expression in Escherichia coli of a thermophilic lipase from Bacillus thermoleovorans ID-1

被引：61
作者：
Cho, AR
Yoo, SK
Kim, EJ
机构：
[1] Yonsei Univ, Coll Engn, Dept Biotechnol, Sudaemun Ku, Seoul 120749, South Korea
[2] Yonsei Univ, Bioprod Res Ctr, Sudaemun Ku, Seoul 120749, South Korea
来源：
FEMS MICROBIOLOGY LETTERS | 2000年 / 186卷 / 02期
关键词：
thermophilic lipase; cloning; overexpression; Bacillus thermoleovorans;
D O I：
10.1111/j.1574-6968.2000.tb09110.x
中图分类号：
Q93 [微生物学];
学科分类号：
071005 ; 100705 ;
摘要：
A thermophilic lipase of Bacillus thermoleovorans ID-1 was cloned and sequenced. The lipase gene codes 416 amino acid residues and contains the conserved pentapeptide Ala-X-Ser-X-Gly as other Bacillus lipase genes. The optimum temperature of the lipase is 75 degrees C. which is higher than other known Bacillus lipases. For expression in Escherichia coli, the lipase gene was subcloned in pET-22b(+) vector with a strong T7 promoter. Lipase activity was approximately 1.4-fold greater than under the native promoter. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
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页码：235 / 238
页数：4
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