Membrane-bound FRET probe visualizes MMP12 activity in pulmonary inflammation

被引：90

作者：

Cobos-Correa, Amanda ^{[1
,2
]}

Trojanek, Johanna B. ^{[3
]}

Diemer, Stefanie ^{[2
,3
]}

Mall, Marcus A. ^{[2
]}

Schultz, Carsten ^{[1
,2
]}

机构：

[1] European Mol Biol Lab, Cell Biol & Biophys Unit, Heidelberg, Germany

[2] European Mol Biol Lab, Mol Med Partnership Unit, Heidelberg, Germany

[3] Univ Heidelberg, Pediat Pulmonol & Cyst Fibrosis Ctr, Dept Pediat 3, Heidelberg, Germany

来源：

NATURE CHEMICAL BIOLOGY | 2009年 / 5卷 / 09期

关键词：

MACROPHAGE METALLOELASTASE; MATRIX METALLOPROTEINASE-12; ACTIVATION; INHIBITORS; EMPHYSEMA; ELASTASE; CELLS; MICE;

D O I：

10.1038/nchembio.196

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

MMP12 is a metalloproteinase implicated in inflammation. To monitor its activity, we synthesized a membrane-targeted reporter (LaRee1) based on Foerster resonance energy transfer (FRET). Unlike existing sensors, LaRee1 detects MMP12 activity by loss of FRET plus internalization of the lipidated fragment. In bronchoalveolar lavages from a mouse model of pulmonary inflammation, LaRee1 detected MMP12 activity at the surface of activated macrophages. LaRee1 may become a powerful tool for monitoring lung disease.

引用

页码：628 / 630

页数：3

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