Nondenaturing polyacrylamide gel electrophoresis to study the dissociation of the p53 MDM2/X complex by potentially anticancer compounds

被引：4

作者：

Sgammato, Roberta ^{[1
,2
]}

Desiderio, Doriana ^{[3
]}

Lamberti, Anna ^{[1
]}

Raimo, Gennaro ^{[3
]}

Novellino, Ettore ^{[4
]}

Carotenuto, Alfonso ^{[4
]}

Masullo, Mariorosario ^{[1
,2
]}

机构：

[1] Univ Naples Parthenope, Dept Movement Sci & Wellness, I-80133 Naples, Italy

[2] CEINGE Biotecnol Avanzate Sc Arl, Naples, Italy

[3] Univ Molise, Dept Biosci & Terr, Pesche, Isernia, Italy

[4] Univ Naples Federico II, Dept Pharm, Naples, Italy

来源：

ELECTROPHORESIS | 2015年 / 36卷 / 24期

关键词：

Complex dissociation; Inhibitors; MDM2; MDMX; p53;

D O I：

10.1002/elps.201500305

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new analytical method to study the dissociation of the complexes between the oncosuppressor p53 and its negative modulators murine double-minute protein 2 (MDM2) or MDMX, is proposed. This technique is reliable to determine the dissociative power exerted by small molecules on the complex taking advantage of the appearance of migrating MDM2 or MDMX in a native polyacrylamide gel, when inhibitors are added to the complex mixture. Therefore, we propose this new approach to easily screen library of compounds, with potential pharmacological anticancer activity.

引用

页码：3101 / 3104

页数：4

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