Polydopamine-assisted functionalization of heparin and vancomycin onto microarc-oxidized 3D printed porous Ti6Al4V for improved hemocompatibility, osteogenic and anti-infection potencies

被引：33

作者：

Zhang, Teng ^{[1
,2
]}

Zhou, Wenhao ^{[3
]}

Jia, Zhaojun ^{[3
,4
]}

Wei, Qingguang ^{[1
,2
]}

Fan, Daoyang ^{[1
,2
]}

Yan, Jianglong ^{[3
]}

Yin, Chuan ^{[1
,2
]}

Cheng, Yan ^{[3
]}

Cai, Hong ^{[1
,2
]}

Liu, Xiaoguang ^{[1
,2
]}

Zhou, Hua ^{[1
,2
]}

Yang, Xiaojie ^{[5
]}

Zheng, Yufeng ^{[3
,4
]}

Liu, Zhongjun ^{[1
,2
]}

机构：

[1] Peking Univ, Dept Orthoped, Hosp 3, Beijing 100191, Peoples R China

[2] Beijing Key Lab Spinal Dis, Beijing 100191, Peoples R China

[3] Peking Univ, Acad Adv Interdisciplinary Studies, Ctr Biomed Mat & Tissue Engn, Beijing 100871, Peoples R China

[4] Peking Univ, Coll Engn, Dept Mat Sci & Engn, Beijing 100871, Peoples R China

[5] Beijing AKEC Med Co Ltd, Beijing 102200, Peoples R China

来源：

SCIENCE CHINA-MATERIALS | 2018年 / 61卷 / 04期

关键词：

3D printing; porous Ti6Al4V; anti-infection; microarc oxidation; osseointegration; vancomycin; SURFACE MODIFICATION; FACILE CONJUGATION; TITANIUM SURFACES; CALCIUM-PHOSPHATE; VERTEBRAL BODY; BONE; BIOCOMPATIBILITY; COATINGS; IMPLANT; INFECTION;

D O I：

10.1007/s40843-017-9208-x

中图分类号：

T [工业技术];

学科分类号：

08 ;

摘要：

Enhanced antiinfection activities, improved hemocompatibility and osteo-compatibility, and reinforced osseointegration are among the most important considerations in designing multifunctional orthopedic biomaterials. Hereby, anti-infective and osteogenic multifunctional 3D printed porous Ti6Al4V implant with excellent hemocompatibility was successfully designed and fabricated. In brief, osteogenic micro-arc oxidation (MAO) coatings with micro/nanoscale porous topography were generated in situ on 3D printed Ti6Al4V scaffolds, on which heparin and vancomycin were easily immobilized. The surface microstructure, morphology, and chemical compositions were characterized employing scanning electron microscopy (SEM), X-ray photoelectron spectroscopy (XPS) and Fourier transform infrared spectroscopy (FTIR). High loading capacity and sustained vancomycin release profiles were revealed using high performance liquid chromatography (HPLC). Favorable antibacterial and antibiofilm performances against pathogenic Staphylococcus aureus (S. aureus) were validated in vitro through microbial viability assays, Live/Dead bacterial staining, and crystal violet staining. Human mesenchymal stem cells (hMSCs) were seeded on the scaffolds and their proliferation and viability were assessed using Cell Counting Kit and Live/Dead cell viability kit. Further, osteoblastic differentiation abilities were evaluated using alkaline phosphatase (ALP) activity as a hall marker. Additionally, the improved hemocompatibility of the heparinized scaffolds was confirmed by activated partial thromboplastin time (APTT), prothrombin time (PT) and thrombin time (TT). Overall, our results show that the surface-modified 3D printed porous Ti6Al4V possesses balanced antibacterial and osteogenic functions while exhibiting extra anticlotting effects, boding well for future application in customized functional reconstruction of intricate bone defects.

引用

页码：579 / 592

页数：14

共 53 条

[1] A calcium-induced signaling cascade leading to osteogenic differentiation of human bone marrow-derived mesenchymal stromal cells [J].

Barradas, Ana M. C. ;

Fernandes, Hugo A. M. ;

Groen, Nathalie ;

Chai, Yoke Chin ;

Schrooten, Jan ;

van de Peppel, Jeroen ;

van Leeuwen, Johannes P. T. M. ;

van Blitterswijk, Clemens A. ;

de Boer, Jan .

BIOMATERIALS, 2012, 33 (11) :3205-3215

[2] Surface delivery of tunable doses of BMP-2 from an adaptable polymeric scaffold induces volumetric bone regeneration [J].

Bouyer, Michael ;

Guillot, Raphael ;

Lavaud, Jonathan ;

Plettinx, Cedric ;

Olivier, Cecile ;

Curry, Veronique ;

Boutonnat, Jean ;

Coll, Jean-Luc ;

Peyrin, Francoise ;

Josserand, Veronique ;

Bettega, Georges ;

Picart, Catherine .

BIOMATERIALS, 2016, 104 :168-181

[3] Biomaterial-Associated Infection: Locating the Finish Line in the Race for the Surface [J].

Busscher, Henk J. ;

van der Mei, Henny C. ;

Subbiahdoss, Guruprakash ;

Jutte, Paul C. ;

van den Dungen, Jan J. A. M. ;

Zaat, Sebastian A. J. ;

Schultz, Marcus J. ;

Grainger, David W. .

SCIENCE TRANSLATIONAL MEDICINE, 2012, 4 (153)

[4] Plasma-Sprayed Ceramic Coatings for Osseointegration [J].

Cao, Huiliang ;

Liu, Xuanyong .

INTERNATIONAL JOURNAL OF APPLIED CERAMIC TECHNOLOGY, 2013, 10 (01) :1-10

[5] The Calgary Biofilm Device: New technology for rapid determination of antibiotic susceptibilities of bacterial biofilms [J].

Ceri, H ;

Olson, ME ;

Stremick, C ;

Read, RR ;

Morck, D ;

Buret, A .

JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (06) :1771-1776

[6]

Cesaro S, 2007, SUPPORT CARE CANCER, V15, P95

[7] The hydrodynamic permeability and surface property of polyethersulfone ultrafiltration membranes with mussel-inspired polydopamine coatings [J].

Cheng, Chong ;

Li, Shuang ;

Zhao, Weifeng ;

Wei, Qiang ;

Nie, Shengqiang ;

Sun, Shudong ;

Zhao, Changsheng .

JOURNAL OF MEMBRANE SCIENCE, 2012, 417 :228-236

[8] Device-associated infections: A macroproblem that starts with microadherence [J].

Darouiche, RO .

CLINICAL INFECTIOUS DISEASES, 2001, 33 (09) :1567-1572

[9] Degradation of polydopamine coatings by sodium hypochlorite: A process depending on the substrate and the film synthesis method [J].

Del Frari, Doriane ;

Bour, Jerome ;

Ball, Vincent ;

Toniazzo, Valerie ;

Ruch, David .

POLYMER DEGRADATION AND STABILITY, 2012, 97 (09) :1844-1849

[10] Chitosan-Recombinamer Layer-by-Layer Coatings for Multifunctional Implants [J].

Govindharajulu, Jeevan Prasaad ;

Chen, Xi ;

Li, Yuping ;

Carlos Rodriguez-Cabello, Jose ;

Battacharya, Mrinal ;

Aparicio, Conrado .

INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2017, 18 (02)

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