Effect of neovestitol-vestitol containing Brazilian red propolis on accumulation of biofilm in vitro and development of dental caries

被引：48

作者：

Bueno-Silva, B. ^{[1
,2
]}

Koo, H. ^{[2
]}

Falsetta, M. L. ^{[2
]}

Alencar, S. M. ^{[3
]}

Ikegaki, M. ^{[4
]}

Rosalen, P. L. ^{[1
]}

机构：

[1] Univ Campinas UNICAMP, Dept Physiol Sci, Piracicaba Dent Sch, Piracicaba, Brazil

[2] Univ Rochester, Ctr Oral Biol & Eastman, Dept Dent, Dept Microbiol & Immunol,Med Ctr, Rochester, NY 14627 USA

[3] Coll Agr Luiz de Queiroz ESALQ USP, Piracicaba, Brazil

[4] Univ Fed Alfenas, Dept Food & Drugs, Sch Pharmaceut Sci, Alfenas, Brazil

来源：

BIOFOULING | 2013年 / 29卷 / 10期

基金：

巴西圣保罗研究基金会;

关键词：

propolis; vestitol; neovestitol; Streptococcus mutans; biofilm; dental caries; COPPER-TRANSPORT OPERON; STREPTOCOCCUS-MUTANS; NATURAL-PRODUCTS; CHEMICAL-COMPOSITION; STRESS TOLERANCE; BOTANICAL ORIGIN; GLUCOSYLTRANSFERASES; EXPRESSION; VIRULENCE; SUCROSE;

D O I：

10.1080/08927014.2013.834050

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The present study examined the influences of the neovestitol-vestitol (NV) containing fraction isolated from Brazilian red propolis on the development of biofilm and expression of virulence factors by Streptococcus mutans using saliva-coated surfaces of hydroxyapatite. In addition, NV was tested in a rodent model of dental caries to assess its potential effectiveness in vivo. Topical applications of NV (800gml(-1)) significantly impaired the accumulation of biofilms of S. mutans by largely disrupting the synthesis of glucosyltransferase-derived exopolysaccharides and the expression of genes associated with the adaptive stress response, such as copYAZ and sloA. Of even greater impact, NV was as effective as fluoride (positive control) in reducing the development of carious lesions in vivo. NV is a promising natural anti-biofilm agent that targets essential virulence traits in S. mutans, which are associated with the formation of cariogenic biofilm and the subsequent onset of dental caries disease.

引用

页码：1233 / 1242

页数：10

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