Identification of the immediate-early genes of white spot syndrome virus

被引：82

作者：

Li, Fang ^{[1
,2
]}

Li, Mingyuan ^{[1
,2
]}

Ke, Wei ^{[1
,2
]}

Ji, Yongchang ^{[1
,2
]}

Bian, Xiaofang ^{[1
,2
]}

Yan, Xiumin ^{[1
]}

机构：

[1] SOA, Inst Oceanog 3, Key Lab Marine Biogenet Resources, Xiamen 361005, Peoples R China

[2] Xiamen Univ, Sch Life Sci, Xiamen 361005, Peoples R China

来源：

VIROLOGY | 2009年 / 385卷 / 01期

基金：

中国国家自然科学基金;

关键词：

WSSV; IE genes; Cycloheximide; Crayfish; Primary Culture; EARLY TRANSCRIPTS; GENOME SEQUENCE; SHRIMP; PROTEIN; ENVELOPE; BZLF1; TRANSACTIVATORS; PATHOGENESIS; PROMOTER; REVEALS;

D O I：

10.1016/j.virol.2008.12.007

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

During viral infection, viral immediate-early (IE) genes encode regulatory proteins critical for the viral life cycle. Here we screened white spot syndrome virus (WSSV) IE genes with cycloheximide (CHX)-treated primary culture of crayfish hemocyte and a WSSV genome tiling microarray. Sixteen ORFs, including a known WSSV IE gene (ie1/wsv069), were identified and confirmed by RT-PCR and time course studies. The 16 identified IE proteins contain four proteins (WSV051. WSV069, WSV100, WSV079) with transcription activity, one (WSV083) with Ser/Thr kinase domain and one (WSV249) previously described to function as an ubiquitin E3 ligase. Furthermore, most of the identified WSSV IE genes cluster in a 14 kb genomic region (WSSV China isolate: 36,052 to 50,300 bp). This type of arrangement may facilitate the coordinate control and rapid expression of IE genes. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：267 / 274

页数：8

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