Production of recombinant proteins in plant cells

被引:7
作者
Gerasimova, S. V. [1 ,2 ]
Smirnova, O. G. [1 ,2 ]
Kochetov, A. V. [1 ,2 ]
Shumnyi, V. K. [1 ,2 ]
机构
[1] Russian Acad Sci, Siberian Branch, Inst Cytol & Genet, Fed Res Ctr, Pr Akad Lavrentyeva 10, Novosibirsk 630090, Russia
[2] Novosibirsk State Univ, Ul Pirogova 2, Novosibirsk 630090, Russia
基金
俄罗斯基础研究基金会;
关键词
higher plants; recombinant protein; glycosylation; promoter; vector; affinity tag; biotechnology; HIGH-LEVEL PRODUCTION; HIGH-YIELD PRODUCTION; MONOCLONAL-ANTIBODY; EXPRESSION SYSTEMS; N-GLYCOSYLATION; TOBACCO CELLS; PURIFICATION; TRANSLATION; PLATFORM; FUSIONS;
D O I
10.1134/S1021443716010076
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Vegetable protein synthesis systems for industry, medicine, and research are becoming increasingly popular. The technology of protein production in plants has certain advantages, compared with the expression systems of bacteria and yeast. The rich variety of promoters, regulatory elements, affinity tags, and fusion partners that are used in molecular biology and plant biotechnology can create hybrid genetic constructs adapted to the solution of various tasks associated with protein synthesis and purification. New methods of modification of plant systems are being developed for the synthesis of functionally active human proteins whose structure is close to the natural analogues. This review shows current approaches to increase the yield of the target protein, facilitating the procedures of its isolation and purification and preventing degradation.
引用
收藏
页码:26 / 37
页数:12
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