HUVEC Tube-formation Assay to Evaluate the Impact of Natural Products on Angiogenesis

被引：38

作者：

Gentile, Maria Teresa ^{[1
]}

Pastorino, Olga ^{[1
]}

Bifulco, Maurizio ^{[2
]}

Colucci-D'Amato, Luca ^{[1
]}

机构：

[1] Univ Campania Luigi Vanvitelli, Lab Cellular & Mol Neuropathol, Dept Environm Biol & Pharmaceut Sci & Technol, Caserta, Italy

[2] Univ Naples Federico II Naples, Dept Mol Med & Biotechnol, Naples, Italy

来源：

JOVE-JOURNAL OF VISUALIZED EXPERIMENTS | 2019年 / 148期

关键词：

Developmental Biology; Issue; 148; Angiogenesis; drug discovery; endothelial cells; ERK; natural compounds; signal transduction; MECHANISMS;

D O I：

10.3791/58591

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Angiogenesis is a phenomenon that includes different processes, such as endothelial cell proliferation, differentiation, and migration, that lead to the formation of new blood vessels and involve several signal transduction pathways. Here we show that the tube formation assay is a simple in vitro method to evaluate the impact of natural products on angiogenesis and to investigate the molecular mechanisms involved. In particular, in the presence of the water extract of Ruta graveolens (RGWE), endothelial cells are no longer able to form a cell-cell network and that the RGWE effects on human umbilical vein endothelial cell (HUVEC) tube formation is abolished by the constitutive activation of MEK.

引用

页数：6

共 13 条

[1] In vitro angiogenesis: endothelial cell tube formation on gelled basement membrane extract [J].