Expressions of the prepro-orexin and orexin type 2 receptor genes in obese rat

被引：12
作者：
Yamamoto, Y
Ueta, Y
Yamashita, H
Asayama, K
Shirahata, A
机构：
[1] Univ Occupat & Environm Hlth, Dept Physiol, Sch Med, Yahatanishi Ku, Kitakyushu, Fukuoka 8078555, Japan
[2] Kyushu Nutr Welfare Univ, Kitakyushu, Fukuoka 8038511, Japan
[3] Univ Occupat & Environm Hlth, Dept Pediat, Sch Med, Kitakyushu, Fukuoka 8078555, Japan
来源：
PEPTIDES | 2002年 / 23卷 / 09期
关键词：
fa/fa; hypothalamus; hypocretin; in situ hybridization histochemistry; orexin; orexin receptors;
D O I：
10.1016/S0196-9781(02)00111-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We examined the expressions of the prepro-orexin gene in the lateral hypothalamic area (LHA), the genes of the neuropeptide Y (NPY) and proopiomelanocortin (POMC) in the arcuate nucleus (ARC), the orexin type 1 receptor (OX1R) gene in the ventromedial hypothalamic nucleus (VMH) and the orexin type 2 receptor (OX2R) gene in the paraventricular nucleus (PVN) in 6-,12- and 18-week-old male lean (Fa/?) and obese (fa/fa) Zucker rats, using in situ hybridization histochemistry. The fa/fa rats showed hyperglycemia at 12- and 18-week-old. The prepro-orexin mRNA level in fa/fa rats at 18-week-old and the OX2R mRNA level in fa/fa rats at 12- and 18-week-old were significantly decreased compared to controls. The NPY mRNA levels in fa/fa rats at each time point were significantly increased compared to controls, but the POMC mRNA levels were decreased. Prepro-orexin and OX2R mRNA levels in fa/fa rats pretreated with insulin normalized to the levels found in Fa/? rats. These results suggest that the regulation of prepro-orexin gene expression might be independent of the regulation of the NPY and POW genes in the ARC in fa/fa rats. (C) 2002 Elsevier Science Inc. All rights reserved.
引用
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页码：1689 / 1696
页数：8
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