Isolation and characterization of novel geldanamycin analogues

被引：17

作者：

Hu, ZH ^{[1
]}

Liu, YQ ^{[1
]}

Tian, ZQ ^{[1
]}

Ma, W ^{[1
]}

Starks, CM ^{[1
]}

Regentin, R ^{[1
]}

Licari, P ^{[1
]}

Myles, DC ^{[1
]}

Hutchinson, CR ^{[1
]}

机构：

[1] Kosan Biosci Inc, Hayward, CA 94545 USA

来源：

JOURNAL OF ANTIBIOTICS | 2004年 / 57卷 / 07期

关键词：

D O I：

10.7164/antibiotics.57.421

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

New geldanamycin analogues with novel structures arising from direct microbial bioconversion and a genetically engineered geldanamycin producer were isolated and characterized. Three compounds, 15-hydroxygeldanamycin, a tricyclic geldanamycin analog (KOSN-1633), and methyl-geldanamycinate(1)), were isolated after geldanamycin was added to a growing culture of the herbimycin producing strain-Streptomyces hygroscopicus AM-3672. Two related compounds, 17-formyl-17-demethoxy-18-O,-21-O-dihydrogeldanamycin and 17-hydroxymethyl-17-demethoxygeldanamycin were isolated from S. hygroscopicus NRRL 3602/pKOS279-78, a geldanamycin-producing strain containing various genes isolated from S. hygroscopicus AM-3672. Compared with geldanamycin, these five new compounds exhibited reduced cytotoxicity against SKBr3 cancer cells.

引用

页码：421 / 428

页数：8

共 15 条

[1] PLASMID CLONING VECTORS FOR THE CONJUGAL TRANSFER OF DNA FROM ESCHERICHIA-COLI TO STREPTOMYCES SPP [J].