Molecular cloning and expression of three estrogen receptors in the protogynous wrasse, Halichoeres trimaculatus

被引：0

作者：

Horiguchi, Ryo ^{[1
]}

Kobayashi, Yasuhisa ^{[1
]}

Nakamura, Masaru ^{[1
]}

机构：

[1] Univ Ryukyus, Trop Biosphere Res Ctr, Sesoko Stn, Okinawa 9050227, Japan

来源：

CYBIUM | 2008年 / 32卷 / 02期

关键词：

Protogynous wrasse; Halichoeres trimaculatus; Estrogen receptor; Cloning; Expression;

D O I：

暂无

中图分类号：

Q95 [动物学];

学科分类号：

071002 ;

摘要：

Estrogens are essential for sex change and behavior in wrasses. To understand the molecular mechanisms of estrogen action in the reproduction of the protogynous wrasse, Halichoeres trimacidatus, we isolated cDNA encoding three estrogen receptor (ER) subtypes from ovary by 5' and 3' rapid amplification cDNA ends (RACE) methods. The deduced amino acid sequence of ER alpha, ER beta 1 and ER beta 2 showed high identity to the red seabream ER alpha (81%), largemouth bass ER beta (82%) and largemouth bass ER gamma (81%), respectively. Real-time polymerase chain reaction revealed some distinctions in tissue expression of ERs, suggesting different functions for each ER subtype in protogynous wrasses.

引用

页码：88 / 89

页数：2