Analysis of salicylic acid based on the fluorescence enhancement of the As(III)-salicylic acid system

A new, simple and sensitive spectrofluorimetric method for the determination of salicylic acid (lambda(ex) = 315 nm, lambda(em) = 408 nm) using As(III) as a sensitizing reagent has been investigated by measuring the increase of fluorescence intensity of salicylic acid due to the complexation of As(III)-salicylic acid in presence of sodium dodecyl sulfate (SDS) 10(-3) M. Under optimum conditions, a significant relationship was obtained between the fluorescence intensity and salicylic acid concentration. A linear calibration curve was obtained in the range 13.8-13812 mu g l(-1) with product-moment correlation coefficient (R) 0.99985 and detection limit 4.2 mu g l(-1). The R.S.D. is 2.35% (n = 5). The method was applied successfully to the determination of salicylic acid in human serum. (c) 2006 Elsevier B.V. All rights reserved.