Controlled SLN Delivery by Thermoresponsive In-situ Forming Lrodible Gels; A Whole-body and Organ Imaging Studyorming Erodible Gels, A Whole-body and Organ Imaging Study

被引:2
作者
Dorraj, Golnar [1 ]
Dadashzadeh, Simin [1 ,2 ]
Erfan, Mohammad [1 ]
Moghimi, Hamid Reza [1 ,2 ]
机构
[1] Shahid Beheshti Univ Med Sci, Sch Pharm, Dept Pharmaceut & Nanotechnol, POB 14155-6153, Tehran, Iran
[2] Shahid Beheshti Univ Med Sci, Pharmaceut Sci Res Ctr, Tehran, Iran
关键词
Controlled release; fluorescence reflectance imaging; in-situ forming gels; solid lipid nanoparticle; SLN; thermoresponsive; tissue imaging; whole-body imaging; SOLID LIPID NANOPARTICLES; PARENTERAL DRUG-DELIVERY; THERMOSENSITIVE HYDROGEL; RELEASE; SYSTEMS; VIVO; BIODISTRIBUTION; LIPOSOMES; VESICLES; BEHAVIOR;
D O I
10.2174/1567201815666180201093424
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
Background: Nanoparticles (NPs) suffer from rapid clearance from body and require frequent dosing if long treatment is required. Method: In order to solve this problem for solid lipid nanoparticles (SLN) and prolong their action, SLNs were incorporated into thermo-responsive Poloxamer sol-gels and their fate was investigated in vivo and in-vitro using a near infrared lipophilic fluorescent dye; dialkylcarbocyanin [1]. Leakage test, release of intact SLNs from sol-gel and SLN size and zeta potential were investigated. Biodistribution of DiR formulations (solution, free SLN and SLN-Gel) was investigated by whole-body and ex-vivo organ imaging after intraperitoneal injection in mice. SLN showed particle size of about 165 nm and a negative zeta potential of about-36 mV. Results: Leakage studies indicated that fluorescent probe does not release from SLNs. Imaging results revealed a steady profile for SLN-Gel over time, while the fluorescence intensity of solution and free SLN showed a burst followed by rapid clearance. Results also showed that SLN release occurs after gel erosion and follows a zero order profile. Conclusion: Our results indicate that NP-incorporated gel can be used to control the release of SLNs from application site and prolong their action in a sustained manner.
引用
收藏
页码:510 / 519
页数:10
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