Metabolomic Analysis of Plasma from GABAB(1) Knock-Out Mice Reveals Decreased Levels of Elaidic Trans-Fatty Acid

被引：1

作者：

Fattuoni, Claudia ^{[1
]}

Barberini, Luigi ^{[2
]}

Noto, Antonio ^{[2
]}

Follesa, Paolo ^{[3
,4
]}

机构：

[1] Univ Cagliari, Dept Chem & Geol Sci, I-09042 Monserrato, Italy

[2] Univ Cagliari, Dept Med Sci & Publ Hlth, I-09042 Monserrato, Italy

[3] Univ Cagliari, Dept Life & Environm Sci, Sect Neurosci & Anthropol, I-09042 Monserrato, Italy

[4] Univ Cagliari, Ctr Excellence Neurobiol Dependence, I-09042 Monserrato, Italy

来源：

METABOLITES | 2020年 / 10卷 / 12期

关键词：

murine model; plasma; metabolomics; GC-MS; elaidic acid; RECEPTORS; ANXIETY; MODULATION; EPILEPSY; CELLS;

D O I：

10.3390/metabo10120484

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Mice lacking the GABA(B(1)) subunit of gamma-aminobutyric acid (GABA) type B receptors exhibit spontaneous seizures, hyperalgesia, hyperlocomotor activity, and memory impairment. Although mice lacking the GABA(B(1)) subunit are viable, they are sterile, and to generate knockout (KO) mice, it is necessary to cross heterozygous (HZ) mice. The aim of our study was to detect the metabolic differences between the three genotypes of GABA(B(1)) KO mice in order to further characterize this experimental animal model. Plasma samples were collected from wild-type (WT), HZ, and KO mice. Samples were analyzed by means of a gas chromatography-mass spectrometry (GC-MS) platform. Univariate t-test, and partial least square discriminant analysis (PLS-DA) were performed to compare the metabolic pattern of different genotypes. The metabolomic analysis highlighted differences between the three genotypes and identified some metabolites less abundant in KO mice, namely elaidic acid and other fatty acids, and chiro-inositol.

引用

页码：1 / 12

页数：12