Development and evaluation of the rVP-ELISA for detection of antibodies against porcine parvovirus

被引：6

作者：

Kong, Miaomiao ^{[1
,2
]}

Peng, Yonggang ^{[1
]}

Cui, Yuchao ^{[1
,3
]}

Chang, Tiecheng ^{[1
,3
]}

Wang, Xiaoling ^{[1
]}

Liu, Zhaoxia ^{[1
]}

Liu, Yonggang ^{[1
]}

Zhu, Yu ^{[1
,2
,3
]}

Luo, Yakun ^{[1
,2
]}

Tang, Qinghai ^{[4
]}

Feng, Li ^{[1
]}

Cui, Shangjin ^{[1
,2
,3
]}

机构：

[1] Chinese Acad Agr Sci, Harbin Vet Res Inst, State Key Lab Vet Biotechnol, Harbin 150001, Heilongjiang, Peoples R China

[2] Chinese Acad Agr Sci, Inst Anim Sci, Beijing 100094, Peoples R China

[3] HLJ August First Land Reclamat Univ, Coll Anim Sci & Technol, Daqing 163319, Peoples R China

[4] Nanyang Normal Univ, Nanyang 473061, Peoples R China

来源：

JOURNAL OF VIROLOGICAL METHODS | 2014年 / 206卷

基金：

中国国家自然科学基金;

关键词：

lnsect-baculovirus system; Porcine parvovirus; rVP2; rVP-ELISA; PIGS; CIRCOVIRUS;

D O I：

10.1016/j.jviromet.2014.06.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The gene encoding the VP2 protein of porcine parvovirus (PPV) was expressed in an insect-baculovirus system. The recombinant (r) VP2 was similar antigenically/functionally to the native capsid protein as demonstrated by hemagglutination (HA), Western blotting using PPV positive sera. The purified rVP2 proteins were used as coating antigen to establish a rVP-ELISA method for detection of PPV positive and negative sera from pigs. The optimal operating conditions of the rVP-ELISA were: the concentration of rVP2 proteins coated on the wells was 2 mu g/mL; the diluted concentration of serum was 1: 150 and that of the enzyme-labeled antibody was 1: 6000. A total of 596 sera were detected by this assay, and the average positive rate was 87%. Compared with France LSI kit, the result showed that the coincidence rate was 96.7%. In conclusion, the rVP2-ELISA is a sensitive and specific method for detecting antibodies against PPV. (C) 2014 Elsevier B.V. All rights reserved.

引用

页码：115 / 118

页数：4

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