Determination of artesunate using reversed-phase HPLC at increased temperature and ELSD detection

被引：10
作者：
Gaudin, Karen [1 ]
Kauss, Tina [1 ]
Lagueny, Anne-Marie [1 ]
Millet, Pascal [1 ]
Fawaz, Fawaz [1 ]
Dubost, Jean-Pierre [1 ]
机构：
[1] Univ Bordeaux 2, Fac Pharm, Chim Analyt Lab, PPF Parasitol Pharm, F-33076 Bordeaux, France
来源：
JOURNAL OF SEPARATION SCIENCE | 2009年 / 32卷 / 02期
关键词：
Artesunate; Dihydroartemisinin; FLSD; Increased temperature HPLC; Rectal formulations; PERFORMANCE LIQUID-CHROMATOGRAPHY; LIGHT-SCATTERING DETECTOR; ARTEMISIA-ANNUA L; ANTIMALARIAL-DRUG; PEDIATRIC USE; HUMAN PLASMA; STABILITY; DIHYDROARTEMISININ; OPTIMIZATION; MEFLOQUINE;
D O I：
10.1002/jssc.200800437
中图分类号：
O65 [分析化学];
学科分类号：
070302 ; 081704 ;
摘要：
Artesunate (ART) determination can be performed by evaporative light scattering detection with mobile phase composed of CH(3)CN/HCOOH 0.01 M (40:60 v/v; pH 2.85). Evaporative light scattering detection instead of ILTV detection allowed to improve the sensitivity and the 1,01). However, the evaporative light scattering detection response of dihydro-artemisinin appears weaker than for ART, whereas with UV detection the response of ART and dihydroartemisinin seemed similar. Constant analysis time was obtained on using the mobile phase with a flow rate of 0.5 mL/min and column temperature at 60 degrees C instead of 0.7 mL/min at room temperature. This led to less solvent consumption. Moreover, decrease in the flow rate and increase in the column temperature were advantageous for higher sensitivity with both evaporative light scattering detection and UV detection. ART determination in rectal gel and suppositories were compared with these different detection modes and similar results were obtained.
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收藏
页码：231 / 237
页数：7
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