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Proteomic Analysis of Embryonic and Young Human Vitreous
被引：16
|作者：
Yee, Kenneth M. P.
[1
,2
]
Feener, Edward P.
[3
]
Madigan, Michele
[4
,5
]
Jackson, Nicholas J.
[6
]
Gao, Ben-Bo
[3
]
Ross-Cisneros, Fred N.
[2
]
Provis, Jan
[7
,8
]
Aiello, Lloyd Paul
[3
,9
]
Sadun, Alfredo A.
[2
,10
]
Sebag, J.
[1
,2
]
机构：
[1] VMR Inst Vitreous Macula Retina, Huntington Beach, CA 92647 USA
[2] Doheny Eye Inst, Los Angeles, CA 90033 USA
[3] Joslin Diabet Ctr, Beetham Eye Inst, Boston, MA 02215 USA
[4] Univ New S Wales, Sch Optometry & Vis Sci, Sydney, NSW, Australia
[5] Univ Sydney, Save Sight Inst, Sydney, NSW 2006, Australia
[6] Univ Calif Los Angeles, David Geffen Sch Med, Dept Med Stat Core, Los Angeles, CA 90095 USA
[7] Australian Natl Univ, John Curtin Sch Med Res, Canberra, ACT 2601, Australia
[8] Australian Natl Univ, Canberra, ACT, Australia
[9] Harvard Univ, Sch Med, Dept Ophthalmol, Boston, MA USA
[10] Univ Calif Los Angeles, David Geffen Sch Med, Dept Ophthalmol, Los Angeles, CA 90095 USA
关键词：
vitreous;
proteomics;
embryology;
vascular development;
DIABETIC-RETINOPATHY;
GEL-ELECTROPHORESIS;
SOLUBLE-PROTEINS;
ANGIOGENESIS;
CATALOG;
MODEL;
HUMOR;
TOOL;
D O I：
10.1167/iovs.15-16809
中图分类号：
R77 [眼科学];
学科分类号：
100212 ;
摘要：
PURPOSE. The proteomic profile of vitreous from second-trimester human embryos and young adults was characterized using mass spectrometry and analyzed for changes in protein levels that may relate to structural changes occurring during this time. This vitreous proteome was compared to previous reports to confirm proteins already identified and reveal novel ones. METHODS. Vitreous from 17 human embryos aged 14 to 20 weeks gestation (WG) and from a 12-, a 14-, a 15-, and a 28-year-old was individually analyzed using tandem mass spectrometry-based proteomics. Peptide spectral count associations with embryonic age were assessed using a general linear model of fold changes and Spearman's rank correlation. Differences between embryonic and young adult vitreous proteomes were also compared. Immunohistochemistry was used to evaluate three proteins in five additional fetal (10-18 WG) human eyes. RESULTS. There were 1217 proteins identified in fetal and young adult human vitreous, 206 after quantile normalization and variance filtering. In embryos, the peptide counts of 37 proteins changed significantly from 14 to 20 WG: 75.7% increased, 24.3% decreased. Immunohistochemistry confirmed the absence of clusterin and cadherin in 10 and 14 WG eyes and their presence at 18 WG. Comparing embryonic to young adult vitreous, 47 proteins were significantly higher or lower. A total of 768 proteins not previously identified in the literature are presented. CONCLUSIONS. Proteins previously unreported in the human vitreous were identified. The human vitreous proteome undergoes significant changes during embryogenesis and young adulthood. A number of protein levels change considerably during the second trimester, with the majority decreasing.
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页码：7036 / 7042
页数：7
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