Effect of Ultra-Strong Static Magnetic Field on Bacteria: Application of Fourier-Transform Infrared Spectroscopy Combined With Cluster Analysis and Deconvolution

被引:12
作者
Hu, Xing [1 ,2 ]
Qiu, Zunan [2 ]
Wang, Yerui [2 ]
She, Zichao [2 ]
Qian, Guangren [2 ]
Ren, Zhongming [1 ]
机构
[1] Shanghai Univ, Sch Mat Sci & Engn, Shanghai 200072, Peoples R China
[2] Shanghai Univ, Sch Environm & Chem Engn, Shanghai 200072, Peoples R China
关键词
DC; magnetic field; spectroscopy; biomacromolecule; FTIR SPECTROSCOPY; STRUCTURAL DISORDERS; IDENTIFICATION; CELL; MICROORGANISMS; SUPPRESSION; DYNAMICS; SPECTRA; STRAIN; DNA;
D O I
10.1002/bem.20506
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
A new method based on Fourier-transform infrared (FTIR) spectroscopy combined with cluster analysis and deconvolution was established to investigate the biological effect of an ultra-strong static magnetic field (SMF) of 10.0 Ton Escherichia coli and Staphylococcus aureus. FTIR spectroscopy was applied to characterize the spectroscopic fingerprints of these bacterial cells with or without the treatment of the SMF. After the calculation, the results of cluster analysis indicated that the SMF had significant effects on E. coli compared with S. aureus, and the effects were reflected by the changes of spectral region of 1500-1200 cm(-1). The deconvolution results of this major indication region showed that the composition and conformation of nucleic acid, protein, and fatty acid of E. coli were altered under the magnetic conditions. Bioelectromagnetics 30:500-507, 2009. (C) 2009 Wiley-Liss, Inc.
引用
收藏
页码:500 / 507
页数:8
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