Spatial and temporal changes of Decorin, Type I collagen and Fibronectin expression in normal and clone bovine placenta

被引：27

作者：

Guillomot, M. ^{[1
]}

Campion, E. ^{[1
]}

Prezelin, A. ^{[1
]}

Sandra, O. ^{[1
]}

Hue, I. ^{[1
]}

Le Bourhis, D. ^{[1
]}

Richard, C. ^{[2
]}

Biase, F. H. ^{[3
]}

Rabel, C. ^{[3
]}

Wallace, R. ^{[4
]}

Lewin, H. ^{[3
,5
,6
]}

Renard, J. -P. ^{[1
]}

Jammes, H. ^{[1
]}

机构：

[1] INRA, Biol Dev & Reprod UMR1198, F-78350 Jouy En Josas, France

[2] INRA, Unite Commune Expt Anim UE1298, F-91630 Leudeville, France

[3] Univ Illinois, Dept Anim Sci, Inst Genom Biol, Urbana, IL 61820 USA

[4] Univ Illinois, Coll Vet Med, Urbana, IL 61820 USA

[5] Univ Calif Davis, Dept Ecol & Evolut, Davis, CA 95616 USA

[6] Univ Calif Davis, Genome Ctr, Davis, CA 95616 USA

来源：

PLACENTA | 2014年 / 35卷 / 09期

关键词：

LEUCINE-RICH PROTEOGLYCANS; EXTRACELLULAR-MATRIX COMPONENTS; ENDOMETRIUM; TROPHOBLAST; MIGRATION; PROLIFERATION; INHIBITION; HYALURONAN; BIGLYCAN; NETWORK;

D O I：

10.1016/j.placenta.2014.06.366

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Introduction: Alteration of expression of various genes including extracellular matrix components, have been suggested to play major role in the placental pathologies after somatic cloning in mammals. The objectives of the present study were to analyze pattern of expression (mRNA and protein) of the small leucine-rich proteoglycan, Decorin in association with Type I Collagen and Fibronectin in bovine placental tissues from normal and clone pregnancies. Methods: Genotyping and allelic expression of Decorin were determined by Sanger sequencing. The expression patterns of Decorin, Type I collagen and Fibronectin 1 were analyzed by quantitative RT-qPCR and combined in situ hybydization (ISH) and immunohistochemistry (IHC) in endometrial and placental tissues from D18 to term from artificially inseminated and somatic cloning pregnancies. Results: The expression levels of DCN increased in the AI endometrial stroma and chorionic mesenchyme during implantation and declined during placentome growth until term. Combined ISH and IHC revealed an unexpected discrepancy mRNA and protein tissue distribution. Moreover, Decorin was maintained in the placentome tissues from SCNT pregnancies while both mRNA and protein were absent in AI derived placenta. Discussion: In bovine, the pattern of expression of Decorin exhibits significant changes during placental formation. Downregulation of Decorin is associated with proliferation, remodeling and vascularization of placental tissues. These observations reinforces the putative role of Decorin in these processes. Conclusions: These observations suggest that Decorin is involved in placental growth and that dysregulation of its expression is associated with placental abnormalities in SCNT derived pregnancy. (C) 2014 Elsevier Ltd. All rights reserved.

引用

页码：737 / 747

页数：11

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