Direct observation of intramolecular hydrogen bonds in peptide 310 helices by 3hJN,C′ scalar couplings

被引：6
作者：
Bellanda, M
Rainaldi, M
Broxterman, QB
Kaptein, B
Formaggio, F
Crisma, M
Mammi, S
Toniolo, C
机构：
[1] Univ Padua, Dept Chem, I-35131 Padua, Italy
[2] CNR ICB, Padova Unit, I-35131 Padua, Italy
[3] Adv Synth & Catalysis, DSM Pharma Chem, NL-6160 MD Geleen, Netherlands
来源：
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2004年 / 43卷 / 24期
关键词：
helical structures; IR spectroscopy; NMR spectroscopy; peptides; X-ray diffraction;
D O I：
10.1002/anie.200454224
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Differentiating helices: The direct observation of hydrogen bonds by 3hJN,C′ scalar couplings (see picture) is not only possible for α-helix and β-sheet peptides and proteins, but for 310-helical peptides as well. The method also provides information on terminal fraying of helices and allows the discrimination between α and 310 helices.
引用
收藏
页码：3152 / 3155
页数：4
相关论文
共 36 条
[1]   The NMR solution structure of the ion channel peptaibol chrysospermin C bound to dodecylphosphocholine micelles [J].
Anders, R ;
Ohlenschläger, O ;
Soskic, V ;
Wenschuh, H ;
Heise, B ;
Brown, LR .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 2000, 267 (06) :1784-1794
[2]   The chemical nature of hydrogen bonding in proteins via NMR:: J-couplings, chemical shifts, and AIM theory [J].
Arnold, WD ;
Oldfield, E .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (51) :12835-12841
[3]  
Bagno A, 2000, CHEM-EUR J, V6, P2925
[4]   Structural dependencies of interresidue scalar coupling h3JNC, and donor 1H chemical shifts in the hydrogen bonding regions of proteins [J].
Barfield, M .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2002, 124 (15) :4158-4168
[5]   CONFORMATIONAL-ANALYSIS OF LINEAR PEPTIDES .5. SPECTROSCOPIC CHARACTERIZATION OF BETA-TURNS IN AIB-CONTAINING OLIGOPEPTIDES IN CHLOROFORM [J].
BONORA, GM ;
MAPELLI, C ;
TONIOLO, C ;
WILKENING, RR ;
STEVENS, ES .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1984, 6 (04) :179-188
[6]   Hydrogen bonds in rubredoxins from mesophilic and hyperthermophilic organisms [J].
Bougault, CM ;
Eidsness, MK ;
Prestegard, JH .
BIOCHEMISTRY, 2003, 42 (15) :4357-4372
[7]  
Burla M. C., 2003, Journal of Applied Crystallography, V36, DOI 10.1107/S0021889803012585
[8]   TERT-BUTYLOXYCARBONYL AND BENZYLOXYCARBONYL AMINO-ACID FLUORIDES - NEW, STABLE RAPID-ACTING ACYLATING AGENTS FOR PEPTIDE-SYNTHESIS [J].
CARPINO, LA ;
MANSOUR, EME ;
SADATAALAEE, D .
JOURNAL OF ORGANIC CHEMISTRY, 1991, 56 (08) :2611-2614
[9]   1-HYDROXY-7-AZABENZOTRIAZOLE - AN EFFICIENT PEPTIDE COUPLING ADDITIVE [J].
CARPINO, LA .
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (10) :4397-4398
[10]   Temperature-dependence properties as studied by of protein hydrogen bond high-resolution NMR [J].
Cordier, F ;
Grzesiek, S .
JOURNAL OF MOLECULAR BIOLOGY, 2002, 317 (05) :739-752
1234