In vivo study of chitosan-natural nano hydroxyapatite scaffolds for bone tissue regeneration

被引：79
作者：
Lee, Jong Seo [1 ]
Baek, Sang Dae [2 ]
Venkatesan, Jayachandran [3 ,4 ]
Bhatnagar, Ira [5 ]
Chang, Hee Kyung [6 ]
Kim, Hui Taek [1 ]
Kim, Se-Kwon [3 ,4 ]
机构：
[1] Pusan Natl Univ Hosp, Dept Orthopaed Surg, Pusan 602739, South Korea
[2] Pusan Natl Univ, Grad Sch, Dept Med, Pusan 602739, South Korea
[3] Pukyong Natl Univ, Dept Marine Bio Convergence Sci, Pusan 608737, South Korea
[4] Pukyong Natl Univ, Marine Bioproc Res Ctr, Pusan 608737, South Korea
[5] Ctr Cellular & Mol Biol, Nanotheranost Lab, Hyderabad 500007, Andhra Pradesh, India
[6] Kosin Univ, Coll Med, Dept Pathol, Pusan 602739, South Korea
来源：
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 2014年 / 67卷
关键词：
Chitosan; Natural hydroxyapatite; Marine biomaterials; COMPOSITE SCAFFOLDS; MINERALIZATION; BIOCERAMICS; PARTICLES;
D O I：
10.1016/j.ijbiomac.2014.03.053
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Significant development has been achieved with bioceramics and biopolymer scaffolds in the construction of artificial bone. In the present study, we have developed and compared chitosan-micro hydroxyapatite (chitosan-mHA) and chitosan-nano hydroxyapatite (chitosan-nHA) scaffolds as bone graft substitutes. The biocompatibility and cell proliferation of the prepared scaffolds were checked with preosteoblast (MC3T3-E1) cells. Total Volume (TV), bone volume (BV), bone surface (BS), trabecular thickness (Tb.Th), trabecular number (Tb.N) and trabecular separation (Tb.Sp) were found to be higher in chitosan-nHA than chitosan-mHA scaffold. Hence, we suggest that chitosan-nHA scaffold could be a promising biomaterial for bone tissue engineering. (C) 2014 Elsevier B.V. All rights reserved.
引用
收藏
页码：360 / 366
页数：7
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