A new highly sensitive and selective Hg(II) probe, 2-(rhodamine-b-hydrazido)-N-(quinolin-8-yl) acetamide (L-1) was developed and characterized. L-1 specifically binds to Hg(II) in the presence of a large excess of other competing ions with visually observable changes in both electronic and fluorescence spectral behaviour to make possible the naked eye detection of Hg(II) at a very low level (up to 4.5 x 10(-7) M) through a fluorescence resonance energy transfer (FRET) process in HEPES buffer (1 mM, pH 7.4; 2% EtOH) at 25 degrees C. The theoretical and experimental kinetic study also support the binding of Hg(II) ion to induce the opening of the spirolactam ring in L-1 for enabling the FRET process. Further studies reveal that the selective dissociation of the L-Hg complex in the presence of sulphide anions to restore the native structure of L-1 is also useful in the detection of sulfide anions with a detection limit of a submicromolar range in the same medium of HEPES buffer (1 mM, pH 7.4; 2% EtOH) at 25 degrees C. L-1 could be employed as a FRET based time dependent reversible chemosensor for imaging Hg(II) in living cells and whole bodies, and also could be used as an imaging probe for the detection of sulfide anions in HeLa cells.
机构:
Karolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, Sweden
Univ Lisbon, Fac Pharm, P-1649003 Lisbon, PortugalKarolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, Sweden
Carvalho, Cristina M. L.
Chew, Eng-Hui
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Karolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, SwedenKarolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, Sweden
Chew, Eng-Hui
Hashemy, Seyed Isaac
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Karolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, SwedenKarolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, Sweden
Hashemy, Seyed Isaac
Lu, Jun
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Karolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, SwedenKarolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, Sweden
机构:
Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea
Ewha Womans Univ, Dept Bioinspired Sci WCU, Seoul 120750, South Korea
Nanjing Univ Technol, Coll Chem & Chem Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Jiangsu, Peoples R ChinaKorea Univ, Dept Chem, Seoul 136701, South Korea
Chen, Xiaoqiang
Pradhan, Tuhin
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Korea Univ, Dept Chem, Seoul 136701, South KoreaKorea Univ, Dept Chem, Seoul 136701, South Korea
Pradhan, Tuhin
Wang, Fang
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Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea
Ewha Womans Univ, Dept Bioinspired Sci WCU, Seoul 120750, South KoreaKorea Univ, Dept Chem, Seoul 136701, South Korea
Wang, Fang
Kim, Jong Seung
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Korea Univ, Dept Chem, Seoul 136701, South KoreaKorea Univ, Dept Chem, Seoul 136701, South Korea
Kim, Jong Seung
Yoon, Juyoung
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Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea
Ewha Womans Univ, Dept Bioinspired Sci WCU, Seoul 120750, South KoreaKorea Univ, Dept Chem, Seoul 136701, South Korea
机构:
Karolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, Sweden
Univ Lisbon, Fac Pharm, P-1649003 Lisbon, PortugalKarolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, Sweden
Carvalho, Cristina M. L.
Chew, Eng-Hui
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机构:
Karolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, SwedenKarolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, Sweden
Chew, Eng-Hui
Hashemy, Seyed Isaac
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机构:
Karolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, SwedenKarolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, Sweden
Hashemy, Seyed Isaac
Lu, Jun
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机构:
Karolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, SwedenKarolinska Inst, Dept Med Biochem & Biophys, Med Nobel Inst Biochem, SE-17177 Stockholm, Sweden
机构:
Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea
Ewha Womans Univ, Dept Bioinspired Sci WCU, Seoul 120750, South Korea
Nanjing Univ Technol, Coll Chem & Chem Engn, State Key Lab Mat Oriented Chem Engn, Nanjing 210009, Jiangsu, Peoples R ChinaKorea Univ, Dept Chem, Seoul 136701, South Korea
Chen, Xiaoqiang
Pradhan, Tuhin
论文数: 0引用数: 0
h-index: 0
机构:
Korea Univ, Dept Chem, Seoul 136701, South KoreaKorea Univ, Dept Chem, Seoul 136701, South Korea
Pradhan, Tuhin
Wang, Fang
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h-index: 0
机构:
Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea
Ewha Womans Univ, Dept Bioinspired Sci WCU, Seoul 120750, South KoreaKorea Univ, Dept Chem, Seoul 136701, South Korea
Wang, Fang
Kim, Jong Seung
论文数: 0引用数: 0
h-index: 0
机构:
Korea Univ, Dept Chem, Seoul 136701, South KoreaKorea Univ, Dept Chem, Seoul 136701, South Korea
Kim, Jong Seung
Yoon, Juyoung
论文数: 0引用数: 0
h-index: 0
机构:
Ewha Womans Univ, Dept Chem & Nano Sci, Seoul 120750, South Korea
Ewha Womans Univ, Dept Bioinspired Sci WCU, Seoul 120750, South KoreaKorea Univ, Dept Chem, Seoul 136701, South Korea