Localization of leptin receptor mRNA splice variants in murine peripheral tissues by RT-PCR and in situ hybridization

被引：265

作者：

Hoggard, N ^{[1
]}

Mercer, JG ^{[1
]}

Rayner, DV ^{[1
]}

Moar, K ^{[1
]}

Trayhurn, P ^{[1
]}

Williams, LM ^{[1
]}

机构：

[1] ROWETT RES INST,MOL NEUROENDOCRINOL GRP,BUCKSBURN AB21 9SB,ABERDEEN,SCOTLAND

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1997年 / 232卷 / 02期

关键词：

D O I：

10.1006/bbrc.1997.6245

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Expression of leptin receptor splice variants, including the long form variant (Ob-Rb), has been examined in murine peripheral tissues. RT-PCR indicates that the leptin receptor, Ob-R, and in particular the Ob-Ra splice variant are expressed in a wide range of tissues. Expression of the Ob-R receptor was localized by in situ hybridization to specific sites in the spleen, testes, kidney, liver, lung, and adrenal. However, the long form leptin receptor, Ob-Rb, was only expressed at significant levels in the medulla of the adrenal and the inner zone of the medulla of the kidney. The specific sites of expression of different splice variants within peripheral tissues has important implications with regard to the function of leptin. (C) 1997 Academic Press.

引用

页码：383 / 387

页数：5

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