Testing gene function early in the B cell lineage in mb1-cre mice

被引:459
作者
Hobeika, E. [1 ]
Thiemann, S. [1 ]
Storch, B. [1 ]
Jumaa, H. [1 ]
Nielsen, P. J. [1 ]
Pelanda, R. [1 ]
Reth, M. [1 ]
机构
[1] Max Planck Inst Immunobiol, D-79108 Freiburg, Germany
关键词
Dnmt1; SRp20; loxP; enhanced yellow fluorescent protein; lymphocyte;
D O I
10.1073/pnas.0605944103
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The mb1 gene encodes the Ig-alpha signaling subunit of the B cell antigen receptor and is expressed exclusively in B cells beginning at the very early pro-B cell stage in the bone marrow. We examine here the efficacy of the mb1 gene as a host locus for cre recombinase expression in B cells. We show that by integrating a humanized cre recombinase into the mb1 locus we obtain extraordinarily efficient recombination of loxP sites in the B cell lineage. The results from a variety of reporter genes including the splicing factor SRp20 and the DNA methylase Dnmt1 suggest that mb1-cre is probably the best model so far described for pan-B cell-specific cre expression. The availability of a mouse line with efficient cre-mediated recombination at an early developmental stage in the B lineage provides an opportunity to study the role of various genes specifically in B cell development and function.
引用
收藏
页码:13789 / 13794
页数:6
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