共 29 条
Testing gene function early in the B cell lineage in mb1-cre mice
被引:459
作者:
Hobeika, E.
[1
]
Thiemann, S.
[1
]
Storch, B.
[1
]
Jumaa, H.
[1
]
Nielsen, P. J.
[1
]
Pelanda, R.
[1
]
Reth, M.
[1
]
机构:
[1] Max Planck Inst Immunobiol, D-79108 Freiburg, Germany
来源:
关键词:
Dnmt1;
SRp20;
loxP;
enhanced yellow fluorescent protein;
lymphocyte;
D O I:
10.1073/pnas.0605944103
中图分类号:
O [数理科学和化学];
P [天文学、地球科学];
Q [生物科学];
N [自然科学总论];
学科分类号:
07 ;
0710 ;
09 ;
摘要:
The mb1 gene encodes the Ig-alpha signaling subunit of the B cell antigen receptor and is expressed exclusively in B cells beginning at the very early pro-B cell stage in the bone marrow. We examine here the efficacy of the mb1 gene as a host locus for cre recombinase expression in B cells. We show that by integrating a humanized cre recombinase into the mb1 locus we obtain extraordinarily efficient recombination of loxP sites in the B cell lineage. The results from a variety of reporter genes including the splicing factor SRp20 and the DNA methylase Dnmt1 suggest that mb1-cre is probably the best model so far described for pan-B cell-specific cre expression. The availability of a mouse line with efficient cre-mediated recombination at an early developmental stage in the B lineage provides an opportunity to study the role of various genes specifically in B cell development and function.
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页码:13789 / 13794
页数:6
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