Enzyme-linked immunosorbent assay to detect Lawsonia intracellularis in rabbits with proliferative enteropathy

被引：14

作者：

Watarai, M ^{[1
]}

Yamato, Y

Horiuchi, N

Kim, S

Omata, Y

Shirahata, T

Furuoka, H

机构：

[1] Obihiro Univ Agr & Vet Med, Dept Appl Vet Sci, Obihiro, Hokkaido 0808555, Japan

[2] Obihiro Univ Agr & Vet Med, Dept Pathol Sci, Obihiro, Hokkaido 0808555, Japan

[3] Obihiro Univ Agr & Vet Med, Dept Vet Basic Sci, Obihiro, Hokkaido 0808555, Japan

来源：

JOURNAL OF VETERINARY MEDICAL SCIENCE | 2004年 / 66卷 / 06期

关键词：

ELISA; Lawsonia intracellularis; rabbit;

D O I：

10.1292/jvms.66.735

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

Lawsonia intracellularis is an obligate intracellular pathogenic bacterium that causes proliferative enteropathy in domestic and experimental animals. Antiserum against synthetic peptides of the Lawsonia surface antigen (LsaA) well recognized L. intracellularis in infected ileum by immunohistochemistry. The synthetic peptides in LsaA showed strong reaction with serum from rabbits infected with L. intracellularis by enzyme-linked immunosorbent assay. These results suggest that ELISA used synthetic peptides in LsaA and anti-LsaA serum might be useful to diagnose for proliferative enteropathy.

引用

页码：735 / 737

页数：3

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