Widefield fluorescence optical sectioning microscopy in a miniature fiber-coupled microscope with active axial scanning

被引：0

作者：

Sanchez, Gabriel Martinez ^{[1
]}

Supekar, Omkar D. ^{[5
,6
]}

Futia, Gregory L. ^{[1
]}

Ozbay, Baris N. ^{[1
]}

Welle, Cristin ^{[4
]}

Bright, Victor M. ^{[6
]}

Gopinath, Juliet T. ^{[5
,7
]}

Restrepo, Diego ^{[3
]}

Shepherd, Douglas ^{[2
,8
]}

Gibson, Emily A. ^{[1
]}

机构：

[1] Univ Colorado, Dept Bioengn, Anschutz Med Campus, Aurora, CO 80045 USA

[2] Univ Colorado, Dept Pharmacol, Anschutz Med Campus, Aurora, CO 80045 USA

[3] Univ Colorado, Dept Cell & Dev Biol, Anschutz Med Campus, Aurora, CO 80045 USA

[4] Univ Colorado, Dept Physiol & Biophys, Anschutz Med Campus, Aurora, CO 80045 USA

[5] Univ Colorado, Dept Elect Comp & Energy Engn, Boulder, CO 80309 USA

[6] Univ Colorado, Dept Mech Engn, Boulder, CO 80309 USA

[7] Univ Colorado, Dept Phys, Boulder, CO 80309 USA

[8] Arizona State Univ, Dept Phys, Tempe, AZ 85287 USA

来源：

2020 CONFERENCE ON LASERS AND ELECTRO-OPTICS (CLEO) | 2020年

关键词：

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

We present widefield structured illumination in a miniature, light-weight fiber-coupled microscope with electrowetting axial scanning. We demonstrate imaging of YFP-labeled neurons in mouse brain tissue showing potential for fast volumetric imaging in freely moving animals. (c) 2020 The Author(s)

引用

页数：2

共 22 条

[1] Two-Photon Imaging of Neuronal Activity in Freely Behaving Mice Using a Miniature Fiber-Coupled Microscope with Active Axial Focusing
Ozbay, Baris N.
Futia, Gregory L.
Ming, M. A.
Hughes, Ethan G.
Restrepo, Diego
Gibson, Emily A.
JOURNAL OF GENERAL PHYSIOLOGY, 2017, 149 (09): : 12A - 12A
[2] Fast Optical Sectioning for Widefield Fluorescence Mesoscopy with the Mesolens based on HiLo Microscopy
Schniete, Jan
Franssen, Aimee
Dempster, John
Bushell, Trevor J.
Amos, William Bradshaw
McConnell, Gail
SCIENTIFIC REPORTS, 2018, 8
[3] Fast Optical Sectioning for Widefield Fluorescence Mesoscopy with the Mesolens based on HiLo Microscopy
Jan Schniete
Aimee Franssen
John Dempster
Trevor J Bushell
William Bradshaw Amos
Gail McConnell
Scientific Reports, 8
[4] Characterization of a fiber-coupled EvenField illumination system for fluorescence microscopy
Berry, Kyla
Taormina, Mike
Maltzer, Zoe
Turner, Kristen
Gorham, Melissa
Nguyen, Thuc
Serafin, Robert
Nicovich, Philip R.
OPTICS EXPRESS, 2021, 29 (15) : 24349 - 24362
[5] Three dimensional two-photon brain imaging in freely moving mice using a miniature fiber coupled microscope with active axial-scanning
Baris N. Ozbay
Gregory L. Futia
Ming Ma
Victor M. Bright
Juliet T. Gopinath
Ethan G. Hughes
Diego Restrepo
Emily A. Gibson
Scientific Reports, 8
[6] Three dimensional two-photon brain imaging in freely moving mice using a miniature fiber coupled microscope with active axial-scanning
Ozbay, Baris N.
Futia, Gregory L.
Ma, Ming
Bright, Victor M.
Gopinath, Juliet T.
Hughes, Ethan G.
Restrepo, Diego
Gibson, Emily A.
SCIENTIFIC REPORTS, 2018, 8
[7] FTTA System Demo Using Optical Fiber-Coupled Active Antennas
Neumann, Niels
Trieb, Robert
Frach, Stephan
Plettemeier, Dirk
PHOTONICS, 2014, 1 (03): : 198 - 210
[8] Axial gain resolution in optical sectioning fluorescence microscopy by shaded-ring filters
Martínez-Corral, M
Ibáñez-López, C
Saavedra, G
OPTICS EXPRESS, 2003, 11 (15): : 1740 - 1745
[9] Application of spectral technique in the scanning probe microscopy: SFOM (scanning fluorescence optical microscope)
Bykov, VA
Feklisov, SG
Saunin, SA
PHYSICS OF LOW-DIMENSIONAL STRUCTURES, 2001, 3-4 : 25 - 30
[10] CONFOCAL MICROSCOPY Dual-detection confocal fluorescence microscope needs no axial scanning
Wallace, John
LASER FOCUS WORLD, 2013, 49 (09): : 21 - +

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