Brushlike Cationic Polymers with Low Charge Density for Gene Delivery

被引：25
作者：
Ahern, Jonathan O'Keeffe [1 ]
Sigen, A. [1 ]
Zhou, Dezhong [1 ]
Gao, Yongsheng [1 ]
Lyu, Jing [1 ]
Meng, Zhao [1 ]
Cutlar, Lara [1 ]
Pierucci, Luca [1 ]
Wang, Wenxin [1 ]
机构：
[1] Univ Coll Dublin, Charles Inst Dermatol, Dublin 4, Ireland
来源：
BIOMACROMOLECULES | 2018年 / 19卷 / 05期
基金：
爱尔兰科学基金会;
关键词：
BRANCHED POLY(BETA-AMINO ESTERS); TRANSFECTION EFFICIENCY; RAFT POLYMERIZATION; TERNARY COMPLEXES; MOLECULAR-WEIGHT; POLYETHYLENIMINE; THERAPY; OPTIMIZATION; COPOLYMERS; STABILITY;
D O I：
10.1021/acs.biomac.7b01267
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Using a combined synthesis approach comprising reversible addition-fragmentation transfer polymerization and ring opening reaction, a series of poly glycidyl methacrylate (polyGMA) polymers were designed and synthesized for gene delivery. These polymers characterized by low cationic charge respective to established gene delivery vectors such as PEI were studied to further elucidate the key structure-activity parameters that mediate efficient and biocompatible gene delivery. Compared to PEI, these brushlike polymers facilitated markedly improved safety and gene delivery efficiency.
引用
收藏
页码：1410 / 1415
页数：6
相关论文
共 38 条
  • [1] Progress of RAFT based polymers in gene delivery
    Ahmed, Marya
    Narain, Ravin
    [J]. PROGRESS IN POLYMER SCIENCE, 2013, 38 (05) : 767 - 790
  • [2] Biophysical characterization of complexation of DNA with block copolymers of poly(2-dimethylaminoethyl) methacrylate, poly(ethylene oxide), and poly(propylene oxide)
    Alvarez-Lorenzo, C
    Barreiro-Iglesias, R
    Concheiro, A
    Iourtchenko, L
    Alakhov, V
    Bromberg, L
    Temchenko, M
    Deshmukh, S
    Hatton, TA
    [J]. LANGMUIR, 2005, 21 (11) : 5142 - 5148
  • [3] Amin ZR, 2013, IRAN J BASIC MED SCI, V16, P150
  • [4] Searching for more effective agents and conditions for the RAFT polymerization of MMA: Influence of dithioester substituents, solvent, and temperature
    Benaglia, M
    Rizzardo, E
    Alberti, A
    Guerra, M
    [J]. MACROMOLECULES, 2005, 38 (08) : 3129 - 3140
  • [5] Poly(glycidyl methacrylate): a highly versatile polymeric building block for post-polymerization modifications
    Benaglia, Massimo
    Alberti, Angelo
    Giorgini, Loris
    Magnoni, Francesco
    Tozzi, Silvia
    [J]. POLYMER CHEMISTRY, 2013, 4 (01) : 124 - 132
  • [6] Highly Branched Poly(β-Amino Esters): Synthesis and Application in Gene Delivery
    Cutlar, Lara
    Zhou, Dezhong
    Gao, Yongsheng
    Zhao, Tianyu
    Greiser, Udo
    Wang, Wei
    Wang, Wenxin
    [J]. BIOMACROMOLECULES, 2015, 16 (09) : 2609 - 2617
  • [7] Breaking down the barriers: siRNA delivery and endosome escape
    Dominska, Monika
    Dykxhoorn, Derek M.
    [J]. JOURNAL OF CELL SCIENCE, 2010, 123 (08) : 1183 - 1189
  • [8] Aminated Poly(glycidyl methacrylate)s for Constructing Efficient Gene Carriers
    Dou, X. B.
    Chai, M. Y.
    Zhu, Y.
    Yang, W. T.
    Xu, F. J.
    [J]. ACS APPLIED MATERIALS & INTERFACES, 2013, 5 (08) : 3212 - 3218
  • [9] Biomedical applications of polymers derived by reversible addition - fragmentation chain-transfer (RAFT)
    Fairbanks, Benjamin D.
    Gunatillake, Pathiraja A.
    Meagher, Laurence
    [J]. ADVANCED DRUG DELIVERY REVIEWS, 2015, 91 : 141 - 152
  • [10] Highly Branched Poly(β-amino esters) for Non-Viral Gene Delivery: High Transfection Efficiency and Low Toxicity Achieved by Increasing Molecular Weight
    Gao, Yongsheng
    Huang, Jian-Yuan
    Ahern, Jonathan O'Keeffe
    Cutlar, Lara
    Zhou, Dezhong
    Lin, Feng-Huei
    Wang, Wenxin
    [J]. BIOMACROMOLECULES, 2016, 17 (11) : 3640 - 3647
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