Ex vivo Culture Assay Using Human Hair Follicles to Study Circadian Characteristics

被引：2

作者：

Nishida, Atsuhiro ^{[1
]}

Miyawaki, Yoshiki ^{[1
]}

Node, Koichi ^{[2
]}

Akashi, Makoto ^{[1
]}

机构：

[1] Yamaguchi Univ, Res Inst Time Studies, Yoshida, Yamaguchi, Japan

[2] Saga Univ, Dept Cardiovasc Med, Nabeshima, Saga, Japan

来源：

BIO-PROTOCOL | 2020年 / 10卷 / 11期

基金：

日本学术振兴会;

关键词：

Circadian rhythm; Clock gene; Human; Hair follicle; Ex vivo; Non-invasive; EXPRESSION;

D O I：

10.21769/BioProtoc.3638

中图分类号：

Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Ex vivo culture assays of biopsy specimens are advantageous for the experimental evaluation of human circadian characteristics. We developed a simple and non-invasive experimental evaluation method for monitoring the expression of circadian clock genes in an ex vivo culture assay using human hair follicles. This method imposes little burden on subjects. This assay is useful for validating correlations between circadian characteristics in hair follicles and intrinsic characteristics observed in physiological and behavioral studies. While they should be further validated, this ex vivo method constitutes a useful tool for estimating in vivo circadian characteristics.

引用

页数：10

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