Integrated Point-of-Care SiNW Biosensors

被引：0

作者：

Azmi, M. A. Mohd ^{[1
,2
]}

Tehrani, Z. ^{[3
]}

Thomas, D. ^{[3
]}

Blayney, G. J. ^{[1
]}

Guy, O. J. ^{[1
]}

机构：

[1] Swansea Univ, Coll Engn, Singleton Pk, Swansea, W Glam, Wales

[2] Univ Kuala Lumpur, British Malaysian Inst BMi, Gombak, Selangor, Malaysia

[3] Swansea Univ, Welsh Ctr Printing & Coating, Swansea, W Glam, Wales

来源：

2014 IEEE SENSORS | 2014年

关键词：

Biosensor; Silicon nanowire; Point-of-care; Nanoimprint; 8-OHdG; SCIL; ELECTRICAL DETECTION;

D O I：

暂无

中图分类号：

TM [电工技术]; TN [电子技术、通信技术];

学科分类号：

0808 ; 0809 ;

摘要：

A SiNW sensor consisting of a SiNW, functionalised with a " bioreceptor" which can interact selectively with a target biomarker, has been developed. By attaching aniline to a SiNW, via coupling with an aryl diazonium salt, the amino group of the aniline molecule has been used to graft antibodies - targeted against 8-hydroxydeoxyguanosine (8OHdG) - onto the SiNW surface. Antibody attachment to SiNW surfaces has been verified using Laser Scanning Confocal Microscopy (LSCM) to detect quantum-dot labelled antibodies bound to the SiNW surface. The current-voltage (I-V) characteristics of the silicon nanowire devices show an increase in resistivity upon chemical functionalization with aniline. A decrease in resistivity is seen on binding of the bioreceptor antibody. The antibody-functionalised SiNW sensor has been used to detect binding of the 8-OHdG biomarker to the SiNW surface within seconds of exposure. SiNW devices have been bonded to a disposable " bio-smartcard" and subsequently slotted into an electronic readout device. This system has been specifically developed for Point-of-Care (POC) use.

引用

页数：4

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