Thyrocyte-specific expression of Cre recombinase in transgenic mice

被引：60
作者：
Kusakabe, T
Kawaguchi, A
Kawaguchi, R
Feigenbaum, L
Kimura, S
机构：
[1] NCI, Lab Metab, Canc Res Ctr, NIH, Bethesda, MD 20892 USA
[2] NCI, SAIC, FCRDC, Transgen Mouse Model Lab, Frederick, MD USA
来源：
GENESIS | 2004年 / 39卷 / 03期
关键词：
cre-mediated recombination; transgenic mouse; thyroid-specific;
D O I：
10.1002/gene.20043
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
A transgenic mouse line that expresses Cre recombinase under control of the human thyroid peroxidase (TPO) gene promoter was established. The activity and specificity of the TPO-driven Cre recombinase were examined by using Northern blotting and by crossing with the ROSA26 reporter transgenic mouse line. In the latter mice, Cre-mediated recombination occurred only in the thyrocytes, and recombination commenced around embryonic day 14.5, at the time during thyroid organogenesis when TPO expression begins. This study demonstrates that the TPO-Cre transgenic mouse is a powerful tool to specifically delete loxP-inserted (floxed) genes in thyrocytes and will be of great value in the study of thyrocyte-specific genes during development and/or in adult thyroids. (C) 2004 Wiley-Liss, Inc.
引用
收藏
页码：212 / 216
页数：5
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