Effect of Vitamins (pyridoxine and nicotinic acid), Thiamine-Hcl and Myo-Inositol at Different Concentrations on Free Amino Acids and Indoles Content of Embryogeinic Callus of in vitro Date Oalm (Sakkoty and Bartamuda Cultivar)

被引：0
作者：
El Sharabasy, Sherif F. [1 ]
Bosila, Hussein A. [2 ]
Abdel-Aal, W. B. [1 ]
Mansour, Bayome M. [2 ]
Bana, Abdel-Monem A. [1 ]
机构：
[1] ARC, Cent Lab Date Palm Res & Dev, Giza, Egypt
[2] Al Azhar Univ, Fac Agr, Floriculture Med & Aromat Plants Dept, Cairo, Egypt
来源：
BY-PRODUCTS OF PALM TREES AND THEIR APPLICATIONS | 2019年 / 11卷
关键词：
vitamins; amino acid; indoles; embryogenic callus; tissue culture; date palm; GROWTH; ENHANCEMENT; CULTURE; IMPROVE;
D O I：
10.21741/9781644900178-20
中图分类号：
TB3 [工程材料学]; TS [轻工业、手工业、生活服务业];
学科分类号：
0805 ; 080502 ; 0822 ;
摘要：
The potential of using tissue culture technique for the production of some bioactive compounds since it allows the manipulation of the biosynthetic routes to increase the production and accumulation of specific compounds. This study was conducted to investigate the effect of vitamins (pyridoxine and nicotinic acid), thiamine-Hcl at different cocentrations (0.5, 1.0 & 2.0 mg/l) and myo-inositol at different concentrations (25, 50, and 100mg/l) at different cocentrations supplemented in MS basal nutrient medium of embryogenic callus of date palm on the production of secondary metabolites of amino acids and indoles. Tow egyption cultivars (Sakkoty and Bartamuda cultivars) of date palm were used. Pyridoxine concentration at 0.5mg/l was the most effective concentration in the production of amino acids and indoles from embryonic callus of the tow studied cultivars of date palm. Nicotinic acid at 0.5mg/l showed also the best results of production of amino acids and indoles from embryogenic callus of two cultivars. Acording to thiamine at 2mg/l concentration was the most effective in inducing the highest significant value of amino acids and indoles from embryonic callus of two cultivars of date palm. Myo-inositol concentration at 25mg/l produced the highest significant value of amino acids and indoles.
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页码：244 / 252
页数：9
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