Bafilomycin A(1) induces apoptosis in PC12 cells independently of intracellular pH

被引：31

作者：

Kinoshita, K ^{[1
]}

Waritani, T ^{[1
]}

Noto, M ^{[1
]}

Takizawa, K ^{[1
]}

Minemoto, Y ^{[1
]}

Nishikawa, Y ^{[1
]}

Ohkuma, S ^{[1
]}

机构：

[1] KANAZAWA UNIV, FAC PHARMACEUT SCI, DEPT BIOCHEM, KANAZAWA, ISHIKAWA 920, JAPAN

来源：

FEBS LETTERS | 1996年 / 398卷 / 01期

关键词：

bafilomycin A(1); apoptosis; vacuolar H+-ATPase; NH4Cl; PC12; cell;

D O I：

10.1016/S0014-5793(96)01182-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PC12 cells growth-arrested with bafilomycin A(1) died showing apoptotic chromatin condensation in the nuclei. The bafilomycin A(1)-induced chromatin condensation was preceded by neurite outgrowth (NOG), required higher concentrations of bafilomycin A(1) than NOG, and was suppressed by cycloheximide and aurintricarboxylic acid. NH4Cl (10 mM), another acidotropic pH perturbing agent, neither induced apoptotic chromatin condensation by itself nor suppressed that induced by bafilomycin A(1), suggesting that bafilomycin A(1)-induced apoptosis occurs independently of intracellular pH in PC12 cells.

引用

页码：61 / 66

页数：6

共 41 条

[1] AURINTRICARBOXYLIC ACID RESCUES PC12 CELLS AND SYMPATHETIC NEURONS FROM CELL-DEATH CAUSED BY NERVE GROWTH-FACTOR DEPRIVATION - CORRELATION WITH SUPPRESSION OF ENDONUCLEASE ACTIVITY [J].

BATISTATOU, A ;

GREENE, LA .

JOURNAL OF CELL BIOLOGY, 1991, 115 (02) :461-471

[2] INDUCTION OF A COMMON PATHWAY OF APOPTOSIS BY STAUROSPORINE [J].

BERTRAND, R ;

SOLARY, E ;

OCONNOR, P ;

KOHN, KW ;

POMMIER, Y .

EXPERIMENTAL CELL RESEARCH, 1994, 211 (02) :314-321

[3] BASIC FIBROBLAST GROWTH-FACTOR ENTERS THE NUCLEOLUS AND STIMULATES THE TRANSCRIPTION OF RIBOSOMAL GENES IN ABAE CELLS UNDERGOING G0-]G1 TRANSITION [J].

BOUCHE, G ;

GAS, N ;

PRATS, H ;

BALDIN, V ;

TAUBER, JP ;

TEISSIE, J ;

AMALRIC, F .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (19) :6770-6774

[4] BAFILOMYCINS - A CLASS OF INHIBITORS OF MEMBRANE ATPASES FROM MICROORGANISMS, ANIMAL-CELLS, AND PLANT-CELLS [J].

BOWMAN, EJ ;

SIEBERS, A ;

ALTENDORF, K .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (21) :7972-7976

[5] GROWTH-INHIBITION OF 3T3 FIBROBLASTS BY LYSOSOMOTROPIC AMINES - CORRELATION WITH EFFECTS ON INTRAVESICULAR PH BUT NOT VACUOLATION [J].

CAIN, CC ;

MURPHY, RF .

JOURNAL OF CELLULAR PHYSIOLOGY, 1986, 129 (01) :65-70

[6] STRUCTURE AND FUNCTION OF VACUOLAR CLASS OF ATP-DRIVEN PROTON PUMPS [J].

FORGAC, M .

PHYSIOLOGICAL REVIEWS, 1989, 69 (03) :765-796

[7] CELL ACIDIFICATION IN APOPTOSIS - GRANULOCYTE-COLONY-STIMULATING FACTOR DELAYS PROGRAMMED CELL-DEATH IN NEUTROPHILS BY UP-REGULATING THE VACUOLAR H+-ATPASE [J].

GOTTLIEB, RA ;

GIESING, HA ;

ZHU, JY ;

ENGLER, RL ;

BABIOR, BM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (13) :5965-5968

[8] Apoptosis induced in Jurkat cells by several agents is preceded by intracellular acidification [J].

Gottlieb, RA ;

Nordberg, J ;

Skowronski, E ;

Babior, BM .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (02) :654-658

[9]

HALL TJ, 1994, CELL BIOL INT, V18, P159

[10]

HEMING TA, 1995, J EXP BIOL, V198, P1711

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