Quantitative analysis of residual protein contamination on reprocessed surgical instruments

被引：49

作者：

Baxter, R. L. ^{[1
]}

Baxter, H. C. ^{[1
]}

Campbell, G. A. ^{[1
]}

Grant, K. ^{[1
]}

Jones, A. ^{[1
]}

Richardson, P. ^{[1
]}

Whittaker, G. ^{[1
]}

机构：

[1] Univ Edinburgh, Sch Chem, Edinburgh EH9 3JJ, Midlothian, Scotland

来源：

JOURNAL OF HOSPITAL INFECTION | 2006年 / 63卷 / 04期

关键词：

cleaning; surgical instruments; prion diseases; sterile services; protein determination;

D O I：

10.1016/j.jhin.2006.03.011

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

'Ready-for-use' instruments from surgical instrument trays were examined after routine Cleaning and sterilization in a blinded study. These reprocessed instruments originated from five National Health Service hospital trust sterile service departments in England and Wales. Determination of residual. protein and peptide contamination was carried out by acid stripping of the instrument surfaces, hydrolysis of the constituent amino acids and quantitative total amino acid analysis. One hundred and twenty instruments were analysed, and the median levels of residual protein contamination per instrument for the individual trays were 267, 260, 163, 456 and 756 mu g. Scanning electron microscopy and energy dispersive X-ray spectroscopic analyses of the instruments showed that tissue deposits were localized on surfaces, but there was no significant correlation between overall protein soiling and instrument complexity. The highest levels of residual contamination were found on instruments used for tonsillectomy and adenoid surgery. (c) 2006 The Hospital Infection Society. Published by Etsevier Ltd. All rights reserved.

引用

页码：439 / 444

页数：6

共 31 条

[1] Worst-case soiling levels for patient-used flexible endoscopes before and after cleaning [J].

Alfa, MJ ;

Degagne, P ;

Olson, N .

AMERICAN JOURNAL OF INFECTION CONTROL, 1999, 27 (05) :392-401

[2] Elimination of transmissible spongiform encephalopathy infectivity and decontamination of surgical instruments by using radio-frequency gas-plasma treatment [J].

Baxter, HC ;

Campbell, GA ;

Whittaker, AG ;

Jones, AC ;

Aitken, A ;

Simpson, AH ;

Casey, M ;

Bountiff, L ;

Gibbard, L ;

Baxter, RL .

JOURNAL OF GENERAL VIROLOGY, 2005, 86 :2393-2399

[3] Swab-based enzyme immunoassay system for detection of meat residues on food contact surfaces as a hygiene monitoring tool [J].

Blais, BW .

JOURNAL OF FOOD PROTECTION, 1999, 62 (04) :386-389

[4]

Bloss R, 2004, J HOSP INFECT, V56, pS44, DOI [10.1016/j.jhin.2003.12.029, 10.1016/j.jhin.20032.12.029]

[5] Iatrogenic Creutzfeldt-Jakob disease at the millennium [J].

Brown, P ;

Preece, M ;

Brandel, JP ;

Sato, T ;

McShane, L ;

Zerr, I ;

Fletcher, A ;

Will, RG ;

Pocchiari, M ;

Cashman, NR ;

d'Aignaux, JH ;

Cervenáková, L ;

Fradkin, J ;

Schonberger, LB ;

Collins, SJ .

NEUROLOGY, 2000, 55 (08) :1075-1081

[6]

Davidson CA, 1999, LUMINESCENCE, V14, P33, DOI 10.1002/(SICI)1522-7243(199901/02)14:1<33::AID-BIO514>3.0.CO

[7]

2-I

[8] London - UK concern over reuse of neurosurgical tools [J].

Dean, M .

LANCET, 2002, 360 (9344) :1485-1485

[9]

DesCoteaux J G, 1995, AORN J, V62, P23, DOI 10.1016/S0001-2092(06)63680-6

[10] Debris on processed ophthalmic instruments: a cause for concern [J].

Dinakaran, S ;

Kayarkar, W .

EYE, 2002, 16 (03) :281-284

← 1 2 3 4 →