Effect of the solid-liquid extraction solvent on the phenolic content and antioxidant activity of three species of Stevia leaves

被引:12
作者
Roberto Medina-Medrano, Jose [1 ]
Estefani Torres-Contreras, Josselin [2 ]
Ignacio Valiente-Banuet, Juan [3 ]
Daniela Mares-Quinones, Maria [3 ]
Vazquez-Sanchez, Monserrat [4 ]
Alvarez-Bernal, Dioselina [5 ]
机构
[1] CONACYT, Inst Politecn Nacl, Ctr Interdisciplinario Invest Desarrollo Integral, Justo Sierra 28, Jiquilpan 59510, Michoacan, Mexico
[2] Univ Cienega Estado Michoacan de Ocampo, Genom Alimentaria, Sahuayo, Mexico
[3] Tecnol Monterrey, Escuela Ingn & Ciencias, Ctr Biotecnol, FEMSA, Monterrey, Mexico
[4] Colegio Postgrad, Posgrad Bot, Carretera Mexico Texcoco Km 36-5, Texcoco 56230, Estado De Mexic, Mexico
[5] Inst Politecn Nacl, Ctr Interdisciplinario Invest Desarrollo Integral, Jiquilpan, Michoacan, Mexico
关键词
Scavenging activity; phenolic compounds; high-performance liquid chromatography with diode array detection (HPLC-DAD); antioxidant capacity; Stevia; FLAVONOIDS; CAPACITY; POLYPHENOLS; GLYCOSIDES; FLOWERS;
D O I
10.1080/01496395.2018.1546741
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
In our study, it was found that solid-liquid extraction solvent influenced the phenolic content in leaf extracts of Stevia origanoides, S. ovata and S. viscida. The highest total phenolic and flavonoids contents, the highest scavenging activity of 2,2-diphenyl-1-picrylhydrazyl (DPPH center dot) radical, and the highest total antioxidant capacity values were observed when 50% ethanol was used. The phenolic compounds identi?ed in the Stevia leaf extracts by high-performance liquid chromatography with diode array detection (HPLC-DAD) include phenolic acids such as chlorogenic, caffeic, p-coumaric and vanillic acid, as well as flavonoids such as quercetin and kaempferol.
引用
收藏
页码:2283 / 2293
页数:11
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