Experimental and simulation study of the wavelength dependent second harmonic generation of collagen in scattering tissues

被引：28

作者：

Hall, Gunnsteinn ^{[1
]}

Tilbury, Karissa B. ^{[1
]}

Campbell, Kirby R. ^{[1
]}

Eliceiri, Kevin W. ^{[1
]}

Campagnola, Paul J. ^{[1
]}

机构：

[1] Univ Wisconsin, Dept Biomed Engn, Madison, WI 53706 USA

来源：

OPTICS LETTERS | 2014年 / 39卷 / 07期

基金：

美国国家科学基金会;

关键词：

EXTRACELLULAR-MATRIX; MICROSCOPY;

D O I：

10.1364/OL.39.001897

中图分类号：

O43 [光学];

学科分类号：

070207 ; 0803 ;

摘要：

We report on the wavelength dependence of second harmonic generation (SHG) of collagen in scattering tissues over the wavelength range of 800-1200 nm. The study incorporates inclusion of the molecular hyperpolarizability beta of collagen and optical scattering, both of which are wavelength dependent. Using 3D SHG imaging and Monte Carlo simulations, we find the wavelength dependence of beta is not well described by a two-state model based on known absorption bands. We further find that longer wavelength excitation is inefficient as the reduction in scattering is overcome by the decreased beta far from resonance and the optimal excitation is within the 800-900 nm range. The impact is larger for backward collected SHG. (C) 2014 Optical Society of America

引用

页码：1897 / 1900

页数：4

共 18 条

[1] THEORETICAL-PI-PI-STAR ABSORPTION, CIRCULAR DICHROIC, AND LINEAR DICHROIC SPECTRA OF COLLAGEN TRIPLE HELICES [J].

CALDWELL, JW ;

APPLEQUIST, J .

BIOPOLYMERS, 1984, 23 (10) :1891-1904

[2] Second harmonic generation microscopy: principles and applications to disease diagnosis [J].

Campagnola, Paul J. ;

Dong, Chen-Yuan .

LASER & PHOTONICS REVIEWS, 2011, 5 (01) :13-26

[3] Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure [J].

Chen, Xiyi ;

Nadiarynkh, Oleg ;

Plotnikov, Sergey ;

Campagnola, Paul J. .

NATURE PROTOCOLS, 2012, 7 (04) :654-669

[4] Simultaneous determination of the second-harmonic generation emission directionality and reduced scattering coefficient from three-dimensional imaging of thick tissues [J].

Hall, Gunnsteinn ;

Eliceiri, Kevin W. ;

Campagnola, Paul J. .

JOURNAL OF BIOMEDICAL OPTICS, 2013, 18 (11)

[5] Goniometric measurements of thick tissue using Monte Carlo simulations to obtain the single scattering anisotropy coefficient [J].

Hall, Gunnsteinn ;

Jacques, Steven L. ;

Eliceiri, Kevin W. ;

Campagnola, Paul J. .

BIOMEDICAL OPTICS EXPRESS, 2012, 3 (11) :2707-2719

[6] Quantitative second harmonic generation imaging of the diseased state osteogenesis imperfecta: Experiment and simulation [J].

LaComb, Ronald ;

Nadiarnykh, Oleg ;

Campagnola, Paul J. .

BIOPHYSICAL JOURNAL, 2008, 94 (11) :4504-4514

[7] Phase matching considerations in second harmonic generation from tissues: Effects on emission directionality, conversion efficiency and observed morphology [J].

LaComb, Ronald ;

Nadiarnykh, Oleg ;

Townsend, Sallie S. ;

Campagnola, Paul J. .

OPTICS COMMUNICATIONS, 2008, 281 (07) :1823-1832

[8] Second-harmonic generation by focused excitation of inhomogeneously distributed scatterers [J].

Mertz, J ;

Moreaux, L .

OPTICS COMMUNICATIONS, 2001, 196 (1-6) :325-330

[9] Alterations of the extracellular matrix in ovarian cancer studied by Second Harmonic Generation imaging microscopy [J].

Nadiarnykh, Oleg ;

LaComb, Ronald B. ;

Brewer, Molly A. ;

Campagnola, Paul J. .

BMC CANCER, 2010, 10

[10] Retention of polarization signatures in SHG microscopy of scattering tissues through optical clearing [J].

Nadiarnykh, Oleg ;

Campagnola, Paul J. .

OPTICS EXPRESS, 2009, 17 (07) :5794-5806

← 1 2 →