A Novel Electroporation System for Living Cell Staining and Membrane Dynamics Interrogation

被引：5

作者：

Zhang, Yuanjun ^{[1
]}

Yan, Zishen ^{[1
]}

Xia, Xingyu ^{[1
]}

Lin, Yuan ^{[2
]}

机构：

[1] HKU Shenzhen Inst Res & Innovat HKU SIRI, Guangzhou 518057, Guangdong, Peoples R China

[2] Univ Hong Kong, Dept Mech Engn, Hong Kong, Peoples R China

来源：

MICROMACHINES | 2020年 / 11卷 / 08期

基金：

中国国家自然科学基金;

关键词：

electroporation; membrane resealing; electrotransfection; live cell staining; cell adhesion; ACTIVATION; MOTILITY; VINCULIN; INVASION; DRIVEN;

D O I：

10.3390/mi11080767

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A novel electroporation system was developed to introduce transient membrane pores to cells in a spatially and temporally controlled manner, allowing us to achieve fast electrotransfection and live cell staining as well as to systematically interrogate the dynamics of the cell membrane. Specifically, using this platform, we showed that both reversible and irreversible electroporation could be induced in the cell population, with nano-sized membrane pores in the former case being able to self-reseal in similar to 10 min. In addition, green fluorescent protein(GFP)-vinculin plasmid and 543 phalloidin have been delivered successively into fibroblast cells, which enables us to monitor the distinct roles of vinculin and F-actin in cell adhesion and migration as well as their possible interplay during these processes. Compared to conventional bulk electroporation and staining methods, the new system offers advantages such as low-voltage operation, cellular level manipulation and testing, fast and adjustable transfection/staining and real-time monitoring; the new system therefore could be useful in different biophysical studies in the future.

引用

页数：11

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