Electrophoretical stretching of DNA in a hybrid microchannel

被引：8
作者：
Zuo Chuncheng [1 ]
Ji Feng [1 ]
Cao Qianqian [1 ]
机构：
[1] Jilin Univ, Coll Mech Sci & Engn, Changchun 130025, Peoples R China
来源：
POLYMER | 2009年 / 50卷 / 22期
关键词：
DNA; Hybrid microchannel; Brownian dynamics; BROWNIAN DYNAMICS SIMULATIONS; EXTENSIONAL FLOW; BEAD-ROD; HYDRODYNAMIC INTERACTIONS; ELONGATIONAL FLOW; SHEAR-FLOW; POLYMER; MOLECULES; DEVICES; CHAIN;
D O I：
10.1016/j.polymer.2009.09.043
中图分类号：
O63 [高分子化学（高聚物）];
学科分类号：
070305 ; 080501 ; 081704 ;
摘要：
The electrophoretical stretching of DNA in a hybrid microchannel is analyzed in this paper. The channel comprises a large insulating cylinder and a hyperbolic contraction that can cause the DNA deformation. Brownian dynamics simulation is used to characterize the dynamical stretching process of a long T4 DNA in hybrid microchannels. The computational results show us the larger average extension of DNA in cylinder-hybridized microchannels than that in the single microcontractions due to the prestretched effect of cylinder on DNA. Moreover, the location and the radius of the insulating cylinder in hybrid microchannels have great effects on the stretching behavior of DNA. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码：5326 / 5332
页数：7
相关论文
共 40 条
  • [1] A magnetic manipulator for studying local rheology and micromechanical properties of biological systems
    Amblard, F
    Yurke, B
    Pargellis, A
    Leibler, S
    [J]. REVIEW OF SCIENTIFIC INSTRUMENTS, 1996, 67 (03) : 818 - 827
  • [2] Aston C, 1999, METHOD ENZYMOL, V303, P55
  • [3] Conformational preconditioning by electrophoresis of DNA through a finite obstacle array
    Balducci, A.
    Doyle, P. S.
    [J]. MACROMOLECULES, 2008, 41 (14) : 5485 - 5492
  • [4] DNA mapping using microfluidic stretching and single-molecule detection of fluorescent site-specific tags
    Chan, EY
    Goncalves, NM
    Haeusler, RA
    Hatch, AJ
    Larson, JW
    Maletta, AM
    Yantz, GR
    Carstea, ED
    Fuchs, M
    Wong, GG
    Gullans, SR
    Gilmanshin, R
    [J]. GENOME RESEARCH, 2004, 14 (06) : 1137 - 1146
  • [5] Numerical study on electrophoretical stretching dynamics of DNA in microcontraction
    Chun-Cheng, Zuo
    Feng, Ji
    Qian-Qian, Cao
    Jing-Song, Yang
    [J]. POLYMER, 2008, 49 (03) : 809 - 815
  • [6] A PADE APPROXIMANT TO THE INVERSE LANGEVIN FUNCTION
    COHEN, A
    [J]. RHEOLOGICA ACTA, 1991, 30 (03) : 270 - 273
  • [7] A microfluidic system for large DNA molecule arrays
    Dimalanta, ET
    Lim, A
    Runnheim, R
    Lamers, C
    Churas, C
    Forrest, DK
    de Pablo, JJ
    Graham, MD
    Coppersmith, SN
    Goldstein, S
    Schwartz, DC
    [J]. ANALYTICAL CHEMISTRY, 2004, 76 (18) : 5293 - 5301
  • [8] Electrokinetic stretching of tethered DNA
    Ferree, S
    Blanch, HW
    [J]. BIOPHYSICAL JOURNAL, 2003, 85 (04) : 2539 - 2546
  • [9] Dynamics of a polymer chain in an elongational flow
    Hofmann, T
    Winkler, RG
    Reineker, P
    [J]. PHYSICAL REVIEW E, 2000, 61 (03): : 2840 - 2847
  • [10] Modeling hydrodynamic interaction in Brownian dynamics: simulations of extensional flows of dilute solutions of DNA and polystyrene
    Hsieh, CC
    Li, L
    Larson, RG
    [J]. JOURNAL OF NON-NEWTONIAN FLUID MECHANICS, 2003, 113 (2-3) : 147 - 191
1234