Photo-lysine captures proteins that bind lysine post-translational modifications

被引：0
|
作者：
Yang, Tangpo [1 ]
Li, Xiao-Meng [1 ]
Bao, Xiucong [1 ]
Fung, Yi Man Eva [1 ]
Li, Xiang David [1 ]
机构：
[1] Univ Hong Kong, Dept Chem, Pokfulam Rd, Hong Kong, Hong Kong, Peoples R China
来源：
NATURE CHEMICAL BIOLOGY | 2016年 / 12卷 / 02期
关键词：
HISTONE; METHYLTRANSFERASE; IDENTIFICATION; PURIFICATION; RECOGNITION; METHYLATION; MECHANISM; CELLS;
D O I：
10.1038/NCHEMBIO.1990
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Post-translational modifications (PTMs) have key roles in regulating protein-protein interactions in living cells. However, it remains a challenge to identify these PTM-mediated interactions. Here we develop a new lysine-based photo-reactive amino acid, termed photo-lysine. We demonstrate that photo-lysine, which is readily incorporated into proteins by native mammalian translation machinery, can be used to capture and identify proteins that recognize lysine PTMs, including 'readers' and 'erasers' of histone modifications.
引用
收藏
页码：70 / +
页数：6
相关论文
共 50 条
  • [31] Determination Of Proteome-wide Post-translational Modifications By Lysine Acetylation In Ischemic Cardiac Cells
    Rajakumar, Aishwarya
    de Lima, Isabela Beatriz
    Gupta, Manish K.
    CIRCULATION RESEARCH, 2022, 131
  • [32] Selective Triazenation Reaction (STaR) of Secondary Amines for Tagging Monomethyl Lysine Post-Translational Modifications
    Nwajiobi, Ogonna
    Mahesh, Sriram
    Streety, Xavier
    Raj, Monika
    ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2021, 60 (13) : 7344 - 7352
  • [33] Metabolism leaves its mark on the powerhouse: recent progress in post-translational modifications of lysine in mitochondria
    Papanicolaou, Kyriakos N.
    O'Rourke, Brian
    Foster, D. Brian
    FRONTIERS IN PHYSIOLOGY, 2014, 5
  • [34] Probing Post-translational Modifications of Amyloidogenic Proteins
    Ge, Ying
    Yang, Jingzhou
    Aprile, Francesco
    EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS, 2023, 52 (SUPPL 1): : S182 - S182
  • [35] Structural Biology of Post-translational Modifications of Proteins
    Kato, Koichi
    YAKUGAKU ZASSHI-JOURNAL OF THE PHARMACEUTICAL SOCIETY OF JAPAN, 2012, 132 (05): : 563 - 573
  • [36] Regulation of DELLA Proteins by Post-translational Modifications
    Blanco-Tourinan, Noel
    Serrano-Mislata, Antonio
    Alabadi, David
    PLANT AND CELL PHYSIOLOGY, 2020, 61 (11) : 1891 - 1901
  • [37] Detecting oxidative post-translational modifications in proteins
    Gianazza, E.
    Crawford, J.
    Miller, I.
    AMINO ACIDS, 2007, 33 (01) : 51 - 56
  • [38] Metaanalysis of post-translational modifications on proteasomal proteins
    Hornbeck, Peter
    FASEB JOURNAL, 2014, 28 (01):
  • [39] Post-translational modifications in the context of therapeutic proteins
    Gary Walsh
    Roy Jefferis
    Nature Biotechnology, 2006, 24 : 1241 - 1252
  • [40] Post-translational modifications in the context of therapeutic proteins
    Walsh, Gary
    Jefferis, Roy
    NATURE BIOTECHNOLOGY, 2006, 24 (10) : 1241 - 1252
12345