Population genetic data from El Salvador (Central america) using AmpF1STR® Identifiler® PCR amplification kit

被引:4
作者
Morales, JA
Monterrosa, JC
Puente, J
机构
[1] Inst Legal Med Dr Roberto Masferrer, Lab Forens Genet, San Salvador, El Salvador
[2] Genom SAU, Dept Human Identificat, Madrid, Spain
来源
PROGRESS IN FORENSIC GENETICS 10 | 2004年 / 1261卷
关键词
STR; DNA polymorphism; El Salvadoran population database; Identifiler((R));
D O I
10.1016/S0531-5131(03)01663-7
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
This study presents allele distribution data of the 13 core short tandem repeat (STR) loci standardized under the Combined DNA Index System (CODIS): CSF1PO, D3S1358, D5S818, D7S820, D8S1179, D13S317, D16S539, D18S51, D21S11, vWA, FGA, TH01, TPOX, and two additional tetranucleotide loci, D2S1338 and D19S433, using AmpFISTR(R) Identifiler(R) PCR Amplification Kit, in an El Salvadoran sample population, consisting of 228 unrelated individuals. All PCR product separations were performed by capillary electrophoresis using an ABI PRISM(R) 310 Genetic Analyzer. GeneScan(R) v 3.7. and Genotyper(R) Software v 3.7 was used for automated STR analysis and allele designation. Allele frequencies for each locus, observed (Ho) and expected (He) heterozygosities, p values of chi square test (p-Chi) and Fisher test (p-Fish) for departures from Hardy-Weinberg expectations (HWE), power of discrimination (PD), probability of exclusion (PE) and Polymorphism Information Content (PIC) were calculated. The results demonstrate that the AmpFISTR(R) Identifiler(R) PCR Amplification Kit is a highly discriminating test suitable for paternity and identity testing in El Salvadoran population. (C) 2003 Elsevier B.V. All rights reserved.
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页码:223 / 225
页数:3
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