Temperature and cryoprotectant influence secondary quinone binding position in bacterial reaction centers

We have determined the first de novo position of the secondary quinone Q(B) in the Rhodobacter sphaeroides reaction center (RC) using phases derived by the single wavelength anomalous dispersion method from crystals with selenomethionine substitution. We found that in frozen RC crystals, Q(B) occupies primarily the proximal binding site. In contrast, our room temperature structure showed that Q(B) is largely in the distal position. Both data sets were collected in dark-adapted conditions. We estimate that the occupancy of the Q(B) site is 80% with a proximal: distal ratio of 4:1 in frozen RC crystals. We could not separate the effect of freezing from the effect of the cryoprotectants ethylene glycol or glycerol. These results could have far-reaching implications in structure/function studies of electron transfer in the acceptor quinone complex because the above are the most commonly used cryoprotectants in spectroscopic experiments. (C) 2004 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.