Functional solubilization of aggregation-prone TRAIL protein facilitated by coexpressing with protein isoaspartate methyltranferase

被引：8

作者：

Zhu, Hu ^{[1
]}

Pan, Ruo-Jun ^{[1
]}

Wang, Tian-Wen ^{[1
]}

Shen, Ya-Ling ^{[1
]}

Wei, Dong-Zhi ^{[1
]}

机构：

[1] E China Univ Sci & Technol, New World Inst Biotechnol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China

来源：

APPLIED MICROBIOLOGY AND BIOTECHNOLOGY | 2006年 / 72卷 / 05期

关键词：

D O I：

10.1007/s00253-006-0383-9

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

TRAIL was a tumor-specific protein in development as a novel anticancer therapeutic agent. Generally, when expressed in recombinant Escherichia coli, TRAIL protein was prone to form inclusion bodies. In this study, coexpression of human TRAIL protein and protein isoaspartate methyltranferase (PIMT) from E. coli on plasmid pBV-TRAIL-PCM in E. coli C600 was investigated to overcome the difficulties in soluble expression. The results showed that this PIMT coexpression strategy exerted a positive effect on the TRAIL protein expression in recombinant E. coli, which led to a mean increase in the intracellular concentration of soluble and total protein of TRAIL by 1.57-fold and 1.33-fold, respectively. At the same time, results also suggested that PIMT was a prospective partner for soluble expression of TRAIL protein.

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页码：1033 / 1038

页数：6

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